Anti-24p3R antibody (ab237539)
Key features and details
- Rabbit polyclonal to 24p3R
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-24p3R antibody -
Description
Rabbit polyclonal to 24p3R -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human 24p3R aa 1-100.
Database link: Q8WUG5 -
Positive control
- WB: U-87 MG whole cell lysate. IHC-P: Human cervical cancer tissue. ICC/IF: A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity greater than 95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab237539 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/50 - 1/500.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/500 - 1/5000. Predicted molecular weight: 57 kDa.
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Notes |
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ICC/IF
1/50 - 1/500. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/500 - 1/5000. Predicted molecular weight: 57 kDa. |
Target
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Function
Cell surface receptor for LCN2 (24p3) that plays a key role in iron homeostasis and transport. Able to bind iron-bound LCN2 (holo-24p3), followed by internalization of holo-24p3 and release of iron, thereby increasing intracellular iron concentration and leading to inhibition of apoptosis. Also binds iron-free LCN2 (apo-24p3), followed by internalization of apo-24p3 and its association with an intracellular siderophore, leading to iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration and resulting in apoptosis. -
Tissue specificity
Expressed in brain. -
Sequence similarities
Belongs to the major facilitator superfamily. Organic cation transporter family. -
Cellular localization
Cell membrane. Vacuole membrane. Upon LCN2-binding, it is internalized. - Information by UniProt
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Database links
- Entrez Gene: 539848 Cow
- Entrez Gene: 51310 Human
- Entrez Gene: 305886 Rat
- Omim: 611461 Human
- SwissProt: Q3SZQ2 Cow
- SwissProt: Q8WUG5 Human
- SwissProt: Q9P290 Rat
- Unigene: 373498 Human
see all -
Alternative names
- 24p3 receptor antibody
- 24p3R antibody
- BOCT antibody
see all
Images
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Anti-24p3R antibody (ab237539) at 1/500 dilution + U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 57 kDa -
Paraffin-embedded human cervical cancer tissue stained for 24p3R using ab237539 at 1/100 dilution in immunohistochemical analysis.
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A549 (Human lung carcinoma cell line) cells stained for 24p3R (green) using ab237539 at 1/100 dilution in ICC/IF, followed by an Alexa-Fluor®488 conjugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab237539 has not yet been referenced specifically in any publications.