Overview

  • Product name

    24(S)-Hydroxycholesterol ELISA Kit
    See all Cholesterol kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Buffer 1 24 28.2ng/ml = 9.3%
    Buffer 2 24 9ng/ml = 5.8%
    Inter-assay
    Sample n Mean SD CV%
    Buffer 1 10 28.6ng/ml = 18.4%
    Buffer 2 10 11.1ng/ml = 18.5%
  • Sample type

    Tissue Culture Media, Tissue Homogenate, Cerebral Spinal Fluid
  • Assay type

    Competitive
  • Sensitivity

    = 0.78 ng/ml
  • Range

    0.39 ng/ml - 100 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Tissue Culture Media = 101.7 % - %
    Tissue Homogenate = 98.6 % - %
    Cerebral Spinal Fluid = 99 % - %

  • Assay time

    2h 00m
  • Assay duration

    Multiple steps standard assay
  • Product overview

    Abcam’s 24(S)-Hydroxycholesterol (24-OHC) kit (ab204530) is an in-vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of 24-OHC levels in tissue culture media, cerebral spinal fluid and tissue homogenate samples.


    The homeostasis and trafficking of cholesterol is an essential component of both the central and peripheral nervous system in the maintenance of neuronal tissues. Disturbances in this homeostasis may be due to the onset of various neurological diseases such as Alzheimer’s Disease, Huntington’s Disease and multiple sclerosis.


    Apolipoprotein E and Cyp46 (also known as 24S-Cholesterol Hydroxylase) are both important in the homeostasis of cerebral cholesterol  6 and thus are of clinical interest in understanding the relation of these molecules with the pathogenesis of these, and potentially other, neurodegenerative diseases.

  • Notes

    24-OHC, an enzymatically-generated side chain-hydroxylated derivative of cholesterol, is a pivotal marker in the study of cerebral cholesterol homeostasis. Cholesterol is unable to cross the blood-brain barrier however, Cyp46 enzyme converts cholesterol to the more soluble 24-OHC, and this hydroxylated form of cholesterol is able to cross the bloodbrain barrier.7,8 This conversion allows for the reduction of cholesterol in the brain and the efflux of 24-OHC from the brain into cerebral spinal fluid and blood. The flux of 24-OHC has been observed in patients with a variety of neurodegenerative diseases. In the instance of Alzheimer’s disease, the change in 24S-hydroxycholesterol concentrations may be indicative of different pathogenetic mechanisms and/or the progression of the disease.3 As in the case of multiple sclerosis, concentrations of 24-OHC have been shown to decrease, likely due to the loss of neuronal cells responsible for the synthesis.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab204530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Images

Protocols

References

This product has been referenced in:

  • Lu F  et al. Upregulation of cholesterol 24-hydroxylase following hypoxia-ischemia in neonatal mouse brain. Pediatr Res 83:1218-1227 (2018). Read more (PubMed: 29718007) »
See 1 Publication for this product

Customer reviews and Q&As

did not work with serum/plasma

Poor Good 4/5 (Ease of Use)
Abreviews
This kit did not work with serum/plasma. Both with or without detection Ab with EDTA plasma showed the same high background levels.

Abcam user community

Verified customer

Submitted Dec 20 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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