Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM3] to 25-OH Vitamin D3
- Suitable for: Competitive ELISA
- Reacts with: Species independent
Product nameAnti-25-OH Vitamin D3 antibody [RM3]
DescriptionRabbit monoclonal [RM3] to 25-OH Vitamin D3
Specificityab219464 reacts to 25-OH Vitamin D3. It does not cross-react with Vitamin D3, Vitamin D2, and 25-OH Vitamin D2.
Tested applicationsSuitable for: Competitive ELISAmore details
Species reactivityReacts with: Species independent
Chemical/ Small Molecule conjugated to bovine serum albumin. (25-OH Vitamin D3 BSA conjugate).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab219464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Competitive ELISA||Use at an assay dependent concentration.
Use 0.5 ng/well – 1.5 ng/well on a 96well plate pre-coated with a goat anti-rabbit IgG polyclonal antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab219464 has been referenced in 2 publications.
- Bespalova O et al. Expression of vitamin D and vitamin D receptor in chorionic villous in missed abortion. Gynecol Endocrinol 35:49-55 (2019). PubMed: 31532316
- Li J et al. Expression of miR-376 in blood of pregnant women with preeclampsia and its effect on 25-hydroxyvitamin D. Exp Ther Med 16:1701-1706 (2018). PubMed: 30186390