Product name25x TBS (pH 7.4)
See all TBS reagents
Tested applicationsSuitable for: IHC-Pmore details
Tris Buffered Saline (TBS) wash buffer ab64248 for use during immunolabeling techniques such as IHC.
In order to provide this product at 25x, the salts in solution are at a very high concentration. It has been found that at temperatures below 44 degrees F (6 degrees C), the salts fall out of solution. If this occurs during your shipment conditions, please stir the product to get the salts back into solution:
Storage instructionsStore at room temperature.
Storage bufferpH: 7.40
Constituents: 22% Sodium chloride, 20.8% Tris HCl
Buffer 25x concentrated. Made from Tris base and HCl.
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RelevanceTBS is a solution of Tris Buffered Saline for use with immunolabeling techniques as a wash buffer. Tween 20 is sometimes added to promote more effective washings, resulting in decreased non-specific background staining. Tween 20 is also known to promote reagent spreading in automated staining methodologies.
- Tris buffered saline
Our Abpromise guarantee covers the use of ab64248 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent dilution.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab64248 has not yet been referenced specifically in any publications.