Overview

  • Product name

    25x TBS (pH 7.4)
    See all TBS reagents
  • Tested applications

    Suitable for: IHC-Pmore details
  • General notes

    Tris Buffered Saline (TBS) wash buffer ab64248 for use during immunolabeling techniques such as IHC.

    In order to provide this product at 25x, the salts in solution are at a very high concentration. It has been found that at temperatures below 44 degrees F (6 degrees C), the salts fall out of solution. If this occurs during your shipment conditions, please stir the product to get the salts back into solution:

Properties

  • Form

    Liquid
  • Storage instructions

    Store at room temperature.
  • Storage buffer

    pH: 7.40
    Constituents: 22% Sodium chloride, 20.8% Tris HCl

    Buffer 25x concentrated. Made from Tris base and HCl.
  • Concentration information loading...
  • Research areas

  • Relevance

    TBS is a solution of Tris Buffered Saline for use with immunolabeling techniques as a wash buffer. Tween 20 is sometimes added to promote more effective washings, resulting in decreased non-specific background staining. Tween 20 is also known to promote reagent spreading in automated staining methodologies.
  • Alternative names

    • Tris buffered saline

Applications

Our Abpromise guarantee covers the use of ab64248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

References

ab64248 has not yet been referenced specifically in any publications.

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