3-Cyano-7-ethoxycoumarin, Fluorogenic cytochrome P450 monooxygenase substrate (ab145344)

Overview

  • Product name

    3-Cyano-7-ethoxycoumarin, Fluorogenic cytochrome P450 monooxygenase substrate
  • Description

    Fluorogenic cytochrome P450 mixed-function monooxygenase substrate.
  • Alternative names

    • 7-Ethoxy-2-oxo-2H-1-benzopyran-3-carbonitrile
    • 7-Ethoxycoumarin-3-carbonitrile
    • CEC
  • Biological description

    Fluoreogenic cytochrome P450 mixed-function monooxygenase substrate. Metabolized to the fluorescent compound 3-cyano-7-hydroxycoumarin. About 50-100 times more sensitive than conventional substrate ethoxy-resorufin due to higher turn over rate.

  • Purity

    > 99%
  • General notes

    Fluorescence: λex 324 nm; λem 414 nm in DMSO.

  • CAS Number

    117620-77-6
  • Chemical structure

    Chemical Structure

Properties

  • Excitation

    324nm
  • Emission

    414nm
  • Molecular weight

    215.20
  • Molecular formula

    C12H9NO3
  • Storage instructions

    Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in DMSO
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Synthetic

References

This product has been referenced in:

  • Pan Y  et al. In vitro effect of important herbal active constituents on human cytochrome P450 1A2 (CYP1A2) activity. Phytomedicine 21:1645-50 (2014). Read more (PubMed: 25442272) »
  • Donato MT  et al. Fluorescence-based assays for screening nine cytochrome P450 (P450) activities in intact cells expressing individual human P450 enzymes. Drug Metab Dispos 32:699-706 (2004). Read more (PubMed: 15205384) »
  • White IN A continuous fluorometric assay for cytochrome P-450-dependent mixed function oxidases using 3-cyano-7-ethoxycoumarin. Anal Biochem 172:304-10 (1988). Read more (PubMed: 3189781) »

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