Key features and details
- Lysine methyltransferase EZH2 inhibitor. Highly potent, competitive s-adenosylhomocysteine hydrolase inhibitor.
- CAS Number: 120964-45-6
- Purity: > 98%
- Soluble in water to 10 mM and in DMSO to 10 mM
- Form / State: Solid
- Source: Synthetic
Product name3-Deazaneplanocin A hydrochloride (DZNep), Lysine methyltransferase EZH2 inhibitor
DescriptionLysine methyltransferase EZH2 inhibitor. Highly potent, competitive s-adenosylhomocysteine hydrolase inhibitor.
- DZNep hydrochloride
Lysine methyltransferase EZH2 inhibitor. Competitive s-adenosylhomocysteine hydrolase inhibitor (Ki = 50 pM). DZNep acts as an epigenetic modifier, specifically blocking trimethylation of lysine 27 on histone H3 and lysine 20 on histone H4. (Miranda et al., Tan et al., Tseng et al.) Induces cancer cell selective apoptosis. Adenosine (ab120498) analog. Inhibits self-renewal of glioblastoma multiforme cancer stem cells (Suva et al.).
Inhibits survival of acute myeloid leukemia blast cells, in combination with a histone deacetylase inhibitor (Fiskus et al.). Enables chemical reprogramming of mouse embryonic fibroblasts iPSC, in combination with CHIR99021 (ab120890), Forskolin (ab120058), Valproic Acid (ab120745), Tranylcypromine (ab120606) and E-616452 (RepSox ab142139), by increasing OCT4 expression at later stages of reprogramming (Hou et al.).
Chemical name5-(4-Aminoimidazo[4,5-c]pyridin-1-yl)-3-(hydroxymethyl)cyclopent-3-ene-1,2-diol hydrochloride
Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in water to 10 mM and in DMSO to 10 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab145628 has been referenced in 1 publication.
- Duncan EJ et al. Genome Architecture Facilitates Phenotypic Plasticity in the Honeybee (Apis mellifera). Mol Biol Evol 37:1964-1978 (2020). PubMed: 32134461