Key features and details
- Rabbit polyclonal to 3-methylcytosine
- Suitable for: Dot blot
- Reacts with: Species independent
- Isotype: IgG
Product nameAnti-3-methylcytosine antibody
DescriptionRabbit polyclonal to 3-methylcytosine
Tested applicationsSuitable for: Dot blotmore details
Species reactivityReacts with: Species independent
Chemical/ Small Molecule corresponding to 3-methylcytosine conjugated to bovine serum albumin.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservatives: 0.05% Sodium azide, 0.05% Proclin 300
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab231795 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
To demonstrate the specifcity of ab231795 a Dot Blot analysis was performed using synthetic oligonucleotides containing different modifed bases.
150 and 50 pmol of the respective oligo’s were spotted on the membrane. ab231795 was diluted 1/400 in PBS-T containing 10 % skimmed milk and 1% BSA.
ab231795 shows a high specificity of the antibody for the oligonucleotide with the 3-methylcytosine modification.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231795 has been referenced in 1 publication.
- Mohan M et al. Human RAD51 paralogue RAD51C fosters repair of alkylated DNA by interacting with the ALKBH3 demethylase. Nucleic Acids Res 47:11729-11745 (2019). PubMed: 31642493