Product nameAnti-3-Nitrotyrosine antibody [2A12]
See all 3-Nitrotyrosine primary antibodies
DescriptionMouse monoclonal [2A12] to 3-Nitrotyrosine
SpecificityThis antibody reacts with 3-Nitrotyrosine
Tested applicationsSuitable for: WBmore details
Species reactivityUbiquitous species reactivity.
3-Nitrotyrosine conjugated to KLH
- Mouse and rat brain lysate.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab61392 as a replacement.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.50
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, 0.328% Sodium phosphate
Concentration information loading...
Purification notesPurified IgG.
Our Abpromise guarantee covers the use of ab52309 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml.|
RelevanceProtein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Various studies have shown that protein tyrosinenitration is limited to specific proteins and that the process is selective. For example, exposure of human surfactant protein A, SP-A, to oxygen-nitrogen intermediates generated by activated alveolar macrophages resulted in specific nitration of SP-A at tyrosines 164 and 166, while addition of 1.2 mMCO 2 resulted in additional nitration at tyrosine 161. The presence of nitrotyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimer’s disease, Parkinson’s disease and amyotrophic lateral sclerosis.
- Nitrotyrosine antibody
All lanes : Anti-3-Nitrotyrosine antibody [2A12] (ab52309) at 1 µg/ml
Lane 1 : mouse brain lysate
Lane 2 : rat brain lysate
Lysates/proteins at 40 µg per lane.
Observed band size: 160,25,55 kDa why is the actual band size different from the predicted?
A: Western Blot using ab52309 detected with Supersignal West Pico Substrate, 30 second exposure.
B: Same experiment blocked with buffer containing 1mM 3-Nitrosine.
This product has been referenced in:
- Jiménez-Altayó F et al. Redox stress in Marfan syndrome: Dissecting the role of the NADPH oxidase NOX4 in aortic aneurysm. Free Radic Biol Med 118:44-58 (2018). WB . Read more (PubMed: 29471108) »
- Himori K et al. Superoxide dismutase/catalase mimetic EUK-134 prevents diaphragm muscle weakness in monocrotalin-induced pulmonary hypertension. PLoS One 12:e0169146 (2017). Read more (PubMed: 28152009) »