Anti-3-Nitrotyrosine antibody [39B6] (ab61392)
Key features and details
- Mouse monoclonal [39B6] to 3-Nitrotyrosine
- Suitable for: IHC-P, WB
- Reacts with: Species independent
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
Anti-3-Nitrotyrosine antibody [39B6]
See all 3-Nitrotyrosine primary antibodies -
Description
Mouse monoclonal [39B6] to 3-Nitrotyrosine -
Host species
Mouse -
Specificity
No detectable crossreactivity with non-nitrated tyrosine. Not species specific. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Full length protein corresponding to 3-Nitrotyrosine. 3-(4-hydroxy-3-nitrophenylacetamido) propionic acid-bovine serum albumin
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Positive control
- SIN-1 (3-morpholinosydnonimine) treated BSA. Use between 1-2.5mM SIN-1 for 1-24 hours, grow cells under normal growth conditions. Please see Yasuda S, Idell S, Liu MC. Biochem J. 2007 Jan 15;401(2):497-503. PMID: 17002600. IHC-P: Mouse back skin and inflammed colon tissue; Human colon carcinoma tissue. Rat liver tissue. WB: A549 cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
39B6 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Corresponding Antibody
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab61392 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (3) |
1/100.
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WB | (6) |
1/1000.
We would recommend a 1:1000 dilution for the antibody, for 2 hours at RT. For blocking, use milk. |
Notes |
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IHC-P
1/100. |
WB
1/1000. We would recommend a 1:1000 dilution for the antibody, for 2 hours at RT. For blocking, use milk. |
Target
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Relevance
Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Various studies have shown that protein tyrosinenitration is limited to specific proteins and that the process is selective. For example, exposure of human surfactant protein A, SP-A, to oxygen-nitrogen intermediates generated by activated alveolar macrophages resulted in specific nitration of SP-A at tyrosines 164 and 166, while addition of 1.2 mMCO 2 resulted in additional nitration at tyrosine 161. The presence of nitrotyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimer’s disease, Parkinson’s disease and amyotrophic lateral sclerosis. -
Alternative names
- 3-Nitrotyrosine antibody
- 3-NT antibody
- Nitrotyrosine antibody
Images
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ab61392 staining 3-nitrotyrosine in Bouin's fixed paraffin-embedded Rat liver tissue at 1/1000 dilution. FITC-conjugated goat anti-mouse IgG(green) was used as a secondary antibody.
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ab61392 staining 3-nitrotyrosine in Bouin's fixed paraffin-embedded backskin sections of transgenic mice.
Blocker: 5% goat serum in buffer. Buffer: 99% washing buffer (7g BSA and 222ul gelatin topped up to 1L dH2O), 1% heat inactivated goat serum. Primary Dilution: 1/100 for 1 hour at room temperature. Secondary: FITC-conjugated goat anti-mouse IgG, 1/50 for 1 hour at room temperature.
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All lanes : Anti-3-Nitrotyrosine antibody [39B6] (ab61392) at 1/3000 dilution
All lanes : Mouse skeletal muscle
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Mouse HRP conjugated at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 4 secondsBlocked with 5% milk for 2 hours.
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Formalin-fixed, paraffin-embedded mouse inflammed colon tissue stained for Nitrotyrosine using ab61392 at 1/1000000 dilution in immunohistochemical analysis.
Secondary Antibody: Biotin Goat Anti-Mouse at 1/2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT.
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Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Nitrotyrosine using ab61392 at 1/25000 dilution in immunohistochemical analysis.
Secondary Antibody: Biotin Goat Anti-Mouse at 1/2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT.
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Anti-3-Nitrotyrosine antibody [39B6] (ab61392) at 1/1000 dilution (2.5 hours at RT with shaking) + A549 (Human lung carcinoma cell line) cell lysate at 15 µg
Secondary
Goat anti-mouse IgG HRP, 1 hour at RT with shaking at 1/1000 dilutionBlock: 5% Skim milk powder in TBST.
Color Development: Chemiluminescent for HRP for 5 mins at RT.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (130)
ab61392 has been referenced in 130 publications.
- Yamaguchi Y et al. Hyperthyroidism exacerbates ischemic reperfusion injury in the kidney. Endocr J 69:263-272 (2022). PubMed: 34629337
- Nagahara T et al. Association between intestinal lymphangiectasia and expression of inducible nitric oxide synthase in dogs with lymphoplasmacytic enteritis. J Vet Med Sci 84:20-24 (2022). PubMed: 34853261
- Cao L et al. Traditional Chinese medicine Lingguizhugan decoction ameliorate HFD-induced hepatic-lipid deposition in mice by inhibiting STING-mediated inflammation in macrophages. Chin Med 17:7 (2022). PubMed: 34983596
- Helwig M et al. Neuronal hyperactivity-induced oxidant stress promotes in vivo α-synuclein brain spreading. Sci Adv 8:eabn0356 (2022). PubMed: 36044566
- Liu X et al. PD-1 Alleviates Cisplatin-Induced Muscle Atrophy by Regulating Inflammation and Oxidative Stress. Antioxidants (Basel) 11:N/A (2022). PubMed: 36139912