Key features and details
- Mouse monoclonal [7A12AF6] to 3-Nitrotyrosine (HRP)
- Suitable for: WB
- Reacts with: Cow, Human
- Conjugation: HRP
- Isotype: IgG2b
Product nameAnti-3-Nitrotyrosine antibody [7A12AF6] (HRP)
See all 3-Nitrotyrosine primary antibodies
DescriptionMouse monoclonal [7A12AF6] to 3-Nitrotyrosine (HRP)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Cow, Human
Predicted to work with: Mouse, Rat
Chemical/ Small Molecule corresponding to 3-Nitrotyrosine. Nitrated KLH (Keyhole Limpet Hemocyanin).
- WB: Nitrated liver homogenate and nitrated bovine serum albumin.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
Purification notesThe antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity >95% by SDS-PAGE.
Light chain typekappa
Our Abpromise guarantee covers the use of ab198491 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
RelevanceProtein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Various studies have shown that protein tyrosinenitration is limited to specific proteins and that the process is selective. For example, exposure of human surfactant protein A, SP-A, to oxygen-nitrogen intermediates generated by activated alveolar macrophages resulted in specific nitration of SP-A at tyrosines 164 and 166, while addition of 1.2 mMCO 2 resulted in additional nitration at tyrosine 161. The presence of nitrotyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimer’s disease, Parkinson’s disease and amyotrophic lateral sclerosis.
- Nitrotyrosine antibody
All lanes : Anti-3-Nitrotyrosine antibody [7A12AF6] (HRP) (ab198491) at 1/5000 dilution
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Nitrated Human Liver Homogenate Standard (ab131380)
Lane 3 : Bovine Serum Albumin
Lane 4 :
Bovine Serum Albumin (nitrated ) (ab131379)
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 1 second
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab198491 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab198491 has not yet been referenced specifically in any publications.