Overview

  • Product name

    Anti-3-Nitrotyrosine antibody [7A12AF6] (HRP)
    See all 3-Nitrotyrosine primary antibodies
  • Description

    Mouse monoclonal [7A12AF6] to 3-Nitrotyrosine (HRP)
  • Host species

    Mouse
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Cow, Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Chemical/ Small Molecule corresponding to 3-Nitrotyrosine. Nitrated KLH (Keyhole Limpet Hemocyanin).

  • Positive control

    • WB: Nitrated liver homogenate and nitrated bovine serum albumin.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab198491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000.

Target

  • Relevance

    Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Various studies have shown that protein tyrosinenitration is limited to specific proteins and that the process is selective. For example, exposure of human surfactant protein A, SP-A, to oxygen-nitrogen intermediates generated by activated alveolar macrophages resulted in specific nitration of SP-A at tyrosines 164 and 166, while addition of 1.2 mMCO 2 resulted in additional nitration at tyrosine 161. The presence of nitrotyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimer’s disease, Parkinson’s disease and amyotrophic lateral sclerosis.
  • Alternative names

    • Nitrotyrosine antibody

Images

  • All lanes : Anti-3-Nitrotyrosine antibody [7A12AF6] (HRP) (ab198491) at 1/5000 dilution

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Nitrated Human Liver Homogenate Standard (ab131380)
    Lane 3 : Bovine Serum Albumin
    Lane 4 : Bovine Serum Albumin (nitrated ) (ab131379)

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 1 second


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab198491 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab198491 has not yet been referenced specifically in any publications.

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