3D Cell Culture Clearing Kit (ab243299)
- Datasheet
- References
- Protocols
Overview
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Product name
3D Cell Culture Clearing Kit
See all Tissue Clearing kits -
Product overview
3D Cell Culture Clearing Kit ab243299 enables the easy set-up of rapid 3D cell culture clearing for 3D imaging using simple protocols and standard laboratory equipment.
This kit can be used with immunostaining, fluorescent proteins and chemical dyes. Clearing is reversible so that you can treat your sample for conventional H&E or IHC staining after 3D imaging.
To help you get started with clearing 3D cell cultures, we have clearing validated antibodies. Validated antibodies are for key markers, such as NeuN, GFAP, Iba1, etc, and are tested for tissue clearing with 1 mm mouse brain sections, and for 3D cell culture clearing with neuronal spheroids and hepatic spheroids (depending on the marker).
If you want to set up clearing of 3D cell cultures on a sample type that we haven’t tested or to use your own antibodies, please consult the protocol book for this kit.
To learn more about tissue clearing and clearing of 3D cell cultures, please review our guide to tissue clearing and clearing 3D cell cultures.
Clearing reagents and companion products are also available as individual products.
These clearing reagents were developed with Visikol Inc, who developed the Visikol® HISTO™ clearing technology which is the basis of our reagents.
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Tested applications
Suitable for: Histologymore details
Properties
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Storage instructions
Please refer to protocols. -
Components 30 ml 15 ml 3D Cell Culture Clearing Reagent 1 x 30ml 1 x 15ml Tissue Clearing Antibody Buffer 1 x 30ml 1 x 15ml Tissue Clearing Blocking Buffer 1 x 30ml 1 x 15ml Tissue Clearing Penetration Buffer 1 x 30ml 1 x 15ml Tissue Clearing Washing Buffer 10X 1 x 70ml 1 x 35ml -
Relevance
The goal for tissue clearing is making large, fixed biological samples transparent. “Large” in this case means thick sections of tissue, whole organoids, entire organs or even entire young rats, ranging in thickness from around 100 µm to several centimeters. Typically, samples of this size are not transparent, and are therefore difficult to analyze using visible wavelengths of light with a microscope. Tissue clearing involves a series of chemical steps that render a large sample transparent.
Applications
Our Abpromise guarantee covers the use of ab243299 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Histology | Use at an assay dependent concentration. |
Images
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P450 antibody used with 3D Cell Culture Clearing Kit and 3D hepatocyte cell cultures -
GFAP antibody ab7260 with 3D Cell Culture Clearing Kit and 3D neuronal spheroid cell cultures
Protocols
References
ab243299 has not yet been referenced specifically in any publications.
