Recombinant
RabMAb

Recombinant Anti-4-1BBL antibody [EPR1172Y] (HRP) (ab199523)

Overview

  • Product name

    Anti-4-1BBL antibody [EPR1172Y] (HRP)
    See all 4-1BBL primary antibodies
  • Description

    Rabbit monoclonal [EPR1172Y] to 4-1BBL (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human 4-1BBL (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HEK293 whole cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab199523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 30 kDa (predicted molecular weight: 27 kDa).

Target

  • Function

    Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages.
  • Tissue specificity

    Expressed in brain, placenta, lung, skeletal muscle and kidney.
  • Sequence similarities

    Belongs to the tumor necrosis factor family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 4 1BB L antibody
    • 4 1BB ligand antibody
    • 4 1BBL antibody
    • 4-1BB ligand antibody
    • 4-1BBL antibody
    • Cd137l antibody
    • Cd157l antibody
    • Homolog of mouse 4 1BB L antibody
    • Homolog of mouse 4 1BBL antibody
    • ILA ligand (TNF related) antibody
    • Ly63l antibody
    • Receptor 4 1BB ligand antibody
    • TNF superfamily member 9 antibody
    • TNFL9_HUMAN antibody
    • Tnfsf9 antibody
    • TNLG5A antibody
    • Tumor necrosis factor (ligand) superfamily member 9 antibody
    • Tumor necrosis factor ligand 5A antibody
    • Tumor necrosis factor ligand superfamily member 9 antibody
    • Tumor necrosis factor superfamily member 9 antibody
    see all

Images

  • Anti-4-1BBL antibody [EPR1172Y] (HRP) (ab199523) at 1/5000 dilution + HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 27 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?


    Exposure time: 12 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab199523 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab199523 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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