Recombinant Anti-4-1BBL antibody [EPR21995-147] (ab254385)
![Western blot - Anti-4-1BBL antibody [EPR21995-147] (ab254385)](https://www.abcam.com/ps/products/254/ab254385/Images/ab254385-396986-anti-41bbl-antibody-epr21995147-western-blot-raji1.jpg "Western blot - Anti-4-1BBL antibody [EPR21995-147] (ab254385)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21995-147] to 4-1BBL
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-4-1BBL antibody [EPR21995-147]
See all 4-1BBL primary antibodies -
Description
Rabbit monoclonal [EPR21995-147] to 4-1BBL -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Raji whole cell lysate. Flow cyt: Raji cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21995-147 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab254385 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 27 kDa).
|
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| Flow Cyt |
1/50.
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| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 27 kDa). |
|
Flow Cyt
1/50. |
Target
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Function
Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages. -
Tissue specificity
Expressed in brain, placenta, lung, skeletal muscle and kidney. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 8744 Human
- Omim: 606182 Human
- SwissProt: P41273 Human
- Unigene: 1524 Human
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Alternative names
- 4 1BB L antibody
- 4 1BB ligand antibody
- 4 1BBL antibody
see all
Images
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Western blot - Anti-4-1BBL antibody [EPR21995-147] (ab254385)All lanes : Anti-4-1BBL antibody [EPR21995-147] (ab254385) at 1/1000 dilution
Lane 1 : Raji (human Burkitts lymphoma cell line) whole cell lysate at 30 µg
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 60 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 27 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression control: K562 (PMID: 20670353).
Exposure time:3 minutes.
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![Flow Cytometry - Anti-4-1BBL antibody [EPR21995-147] (ab254385)](https://www.abcam.com/ps/products/254/ab254385/Images/ab254385-1-anti-41bbl-antibody-epr21995147-flow-cytometry-raji1.jpg)
Flow Cytometry - Anti-4-1BBL antibody [EPR21995-147] (ab254385)Flow cytometric analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labelling 4-1BBL with ab254385 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
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![Anti-4-1BBL antibody [EPR21995-147] (ab254385)](https://www.abcam.com/ps/products/254/ab254385/Images/ab254385-3-benefits-of-recombinant-antibodies.png)
Anti-4-1BBL antibody [EPR21995-147] (ab254385)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab254385 has not yet been referenced specifically in any publications.
