Recombinant Anti-4-1BBL antibody [EPR21995-147] (ab254385)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21995-147] to 4-1BBL
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-4-1BBL antibody [EPR21995-147]
See all 4-1BBL primary antibodies -
Description
Rabbit monoclonal [EPR21995-147] to 4-1BBL -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Raji whole cell lysate. Flow cyt: Raji cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21995-147 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab254385 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 27 kDa).
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Flow Cyt |
1/50.
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Notes |
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WB
1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 27 kDa). |
Flow Cyt
1/50. |
Target
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Function
Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages. -
Tissue specificity
Expressed in brain, placenta, lung, skeletal muscle and kidney. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 8744 Human
- Omim: 606182 Human
- SwissProt: P41273 Human
- Unigene: 1524 Human
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Alternative names
- 4 1BB L antibody
- 4 1BB ligand antibody
- 4 1BBL antibody
see all
Images
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All lanes : Anti-4-1BBL antibody [EPR21995-147] (ab254385) at 1/1000 dilution
Lane 1 : Raji (human Burkitts lymphoma cell line) whole cell lysate at 30 µg
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 60 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 27 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression control: K562 (PMID: 20670353).
Exposure time:3 minutes.
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Flow cytometric analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labelling 4-1BBL with ab254385 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab254385 has not yet been referenced specifically in any publications.