Product name4-Di-2-ASP, Cationic mitochondrial dye
DescriptionCationic mitochondrial dye used for staining presynaptic nerve terminals independent of neuronal activity.
- 4-(4-Diethylaminostyryl)-1-methylpyridinium iodide
- 4-(4-diethylaminostyryl)-N-methylpyridinium iodide
Photostable dye, which is nontoxic to cells and has been employed to stain living nerve terminals in rabbit corneal epithelium, in rat epidermis and at mouse, snake and frog neuromuscular junctions. Methods for using 4-Di-2-ASP to image neuronal cells in live animals have been described.
Fluorescence: λex 485 nm; λem 603 nm in methanol.
Chemical name4-(4-Diethylaminostyryl)-1-methylpyridinium iodide
Storage instructionsStore at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in DMSO
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
This product has been referenced in:
- Wilkinson RS & Lunin SD Properties of "reconstructed" motor synapses of the garter snake. J Neurosci 14:3319-32 (1994). Read more (PubMed: 8182475) »
- Chen L & Ko CP Extension of synaptic extracellular matrix during nerve terminal sprouting in living frog neuromuscular junctions. J Neurosci 14:796-808 (1994). Read more (PubMed: 8301362) »
- MacIver MB & Tanelian DL Structural and functional specialization of A delta and C fiber free nerve endings innervating rabbit corneal epithelium. J Neurosci 13:4511-24 (1993). Read more (PubMed: 8410200) »
- Herrera AA & Banner LR The use and effects of vital fluorescent dyes: observation of motor nerve terminals and satellite cells in living frog muscles. J Neurocytol 19:67-83 (1990). Read more (PubMed: 2351996) »
- Nurse CA & Farraway L Characterization of Merkel cells and mechanosensory axons of the rat by styryl pyridinium dyes. Cell Tissue Res 255:125-8 (1989). Read more (PubMed: 2472215) »
- Magrassi L et al. Fluorescent probes that stain living nerve terminals. J Neurosci 7:1207-14 (1987). Read more (PubMed: 3572476) »