Key features and details
- Cationic mitochondrial dye used for staining presynaptic nerve terminals independent of neuronal activity.
- CAS Number: 105802-46-8
- Purity: > 97%
- Soluble in DMSO
- Form / State: Solid
- Source: Synthetic
Product name4-Di-2-ASP, Cationic mitochondrial dye
DescriptionCationic mitochondrial dye used for staining presynaptic nerve terminals independent of neuronal activity.
- 4-(4-Diethylaminostyryl)-1-methylpyridinium iodide
- 4-(4-diethylaminostyryl)-N-methylpyridinium iodide
Photostable dye, which is nontoxic to cells and has been employed to stain living nerve terminals in rabbit corneal epithelium, in rat epidermis and at mouse, snake and frog neuromuscular junctions. Methods for using 4-Di-2-ASP to image neuronal cells in live animals have been described.
Fluorescence: λex 485 nm; λem 603 nm in methanol.
Chemical name4-(4-Diethylaminostyryl)-1-methylpyridinium iodide
Storage instructionsStore at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in DMSO
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab145266 has been referenced in 1 publication.
- Wright CM et al. scRNA-Seq Reveals New Enteric Nervous System Roles for GDNF, NRTN, and TBX3. Cell Mol Gastroenterol Hepatol 11:1548-1592.e1 (2021). PubMed: 33444816