Product nameAnti-4-Hydroxy-2-hexenal antibody [6F10]
See all 4-Hydroxy-2-hexenal primary antibodies
DescriptionMouse monoclonal [6F10] to 4-Hydroxy-2-hexenal
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Species independent
Chemical/ Small Molecule corresponding to 4-Hydroxy-2-hexenal conjugated to keyhole limpet haemocyanin.
- WB: 4-hydroxyl nonenal-BSA, and 4-hydroxy-2-hexenal-BSA. Flow cyt: SH-SY5Y cells (treated with hydrogen peroxide).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Purification notesPurified from TCS.
Our Abpromise guarantee covers the use of ab243068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Relevance4-Hydroxy-2-alkenals are major lipid peroxidation products, and show many biological effects such as high toxicity to cells. 4-Hydroxy-2-hexenal (HHE) is an aldehyde formed during peroxidation of n-3 fatty acids such as docosahexaenoic acid. HHE is a highly reactive aldehyde and reacts with the histidine residues of proteins to form Michael-addition type adducts.
- HHE antibody
All lanes : Anti-4-Hydroxy-2-hexenal antibody [6F10] (ab243068) at 1/1000 dilution
Lane 1 : BSA at 0.5 µg
Lane 2 : 4-hydroxyl nonenal-BSA at 0.5 µg
Lane 3 : 4-hydroxy nonenal-BSA at 2 µg
Lane 4 : 4-hydroxy-2-hexenal BSA at 0.5 µg
Lane 5 : 4-hydroxy-2-hexenal BSA at 2 µg
All lanes : Goat Anti-Mouse IgG: HRP at 1/2000 dilution
Developed using the ECL technique.
Exposure time: 5 minutes
Primary incubation for 2 hours at room temperature.
Secondary incubation for 1 hour at room temperature.
Block: 5% Skimmed milk in TBST.
Flow cytometric analysis of 90% methanol-fixed SH-SY5Y (human neuroblastoma cell line from bone marrow) cells (+ 250 μM hydrogen peroxide treatment for 24 hours) labeling 4-hydroxy-2-hexenal with ab243068 at 1/100 dilution for 30 minutes on ice (solid line) compared with an Isotype control IgG (dashed line). A Goat Anti-Mouse: PE at 1/100 dilution was used as the secondary antibody.
ab243068 has not yet been referenced specifically in any publications.