Overview

  • Product name

    Anti-4-Hydroxy-2-hexenal antibody [6F10]
    See all 4-Hydroxy-2-hexenal primary antibodies
  • Description

    Mouse monoclonal [6F10] to 4-Hydroxy-2-hexenal
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule corresponding to 4-Hydroxy-2-hexenal conjugated to keyhole limpet haemocyanin.

  • Positive control

    • WB: 4-hydroxyl nonenal-BSA, and 4-hydroxy-2-hexenal-BSA. Flow cyt: SH-SY5Y cells (treated with hydrogen peroxide).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from TCS.
  • Clonality

    Monoclonal
  • Clone number

    6F10
  • Isotype

    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab243068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
Flow Cyt 1/50.

Target

  • Relevance

    4-Hydroxy-2-alkenals are major lipid peroxidation products, and show many biological effects such as high toxicity to cells. 4-Hydroxy-2-hexenal (HHE) is an aldehyde formed during peroxidation of n-3 fatty acids such as docosahexaenoic acid. HHE is a highly reactive aldehyde and reacts with the histidine residues of proteins to form Michael-addition type adducts.
  • Alternative names

    • HHE antibody

Images

  • All lanes : Anti-4-Hydroxy-2-hexenal antibody [6F10] (ab243068) at 1/1000 dilution

    Lane 1 : BSA at 0.5 µg
    Lane 2 : 4-hydroxyl nonenal-BSA at 0.5 µg
    Lane 3 : 4-hydroxy nonenal-BSA at 2 µg
    Lane 4 : 4-hydroxy-2-hexenal BSA at 0.5 µg
    Lane 5 : 4-hydroxy-2-hexenal BSA at 2 µg

    Secondary
    All lanes : Goat Anti-Mouse IgG: HRP at 1/2000 dilution

    Developed using the ECL technique.

    Exposure time: 5 minutes


    Primary incubation for 2 hours at room temperature.

    Secondary incubation for 1 hour at room temperature.

    Block: 5% Skimmed milk in TBST.

  • Flow cytometric analysis of 90% methanol-fixed SH-SY5Y (human neuroblastoma cell line from bone marrow) cells (+ 250 μM hydrogen peroxide treatment for 24 hours) labeling 4-hydroxy-2-hexenal with ab243068 at 1/100 dilution for 30 minutes on ice (solid line) compared with an Isotype control IgG (dashed line). A Goat Anti-Mouse: PE at 1/100 dilution was used as the secondary antibody.

References

ab243068 has not yet been referenced specifically in any publications.

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