Key features and details
- Mouse monoclonal [6F10] to 4-Hydroxy-2-hexenal
- Suitable for: WB, Flow Cyt
- Reacts with: Species independent
- Isotype: IgG2b
Product nameAnti-4-Hydroxy-2-hexenal antibody [6F10]
See all 4-Hydroxy-2-hexenal primary antibodies
DescriptionMouse monoclonal [6F10] to 4-Hydroxy-2-hexenal
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Species independent
Chemical/ Small Molecule corresponding to 4-Hydroxy-2-hexenal conjugated to keyhole limpet haemocyanin.
- WB: 4-hydroxyl nonenal-BSA, and 4-hydroxy-2-hexenal-BSA. Flow cyt: SH-SY5Y cells (treated with hydrogen peroxide).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Purification notesPurified from TCS.
Our Abpromise guarantee covers the use of ab243068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Relevance4-Hydroxy-2-alkenals are major lipid peroxidation products, and show many biological effects such as high toxicity to cells. 4-Hydroxy-2-hexenal (HHE) is an aldehyde formed during peroxidation of n-3 fatty acids such as docosahexaenoic acid. HHE is a highly reactive aldehyde and reacts with the histidine residues of proteins to form Michael-addition type adducts.
- HHE antibody
All lanes : Anti-4-Hydroxy-2-hexenal antibody [6F10] (ab243068) at 1/1000 dilution
Lane 1 : BSA at 0.5 µg
Lane 2 : 4-hydroxyl nonenal-BSA at 0.5 µg
Lane 3 : 4-hydroxy nonenal-BSA at 2 µg
Lane 4 : 4-hydroxy-2-hexenal BSA at 0.5 µg
Lane 5 : 4-hydroxy-2-hexenal BSA at 2 µg
All lanes : Goat Anti-Mouse IgG: HRP at 1/2000 dilution
Developed using the ECL technique.
Exposure time: 5 minutes
Primary incubation for 2 hours at room temperature.
Secondary incubation for 1 hour at room temperature.
Block: 5% Skimmed milk in TBST.
Flow cytometric analysis of 90% methanol-fixed SH-SY5Y (human neuroblastoma cell line from bone marrow) cells (+ 250 μM hydrogen peroxide treatment for 24 hours) labeling 4-hydroxy-2-hexenal with ab243068 at 1/100 dilution for 30 minutes on ice (solid line) compared with an Isotype control IgG (dashed line). A Goat Anti-Mouse: PE at 1/100 dilution was used as the secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab243068 has not yet been referenced specifically in any publications.