Key features and details
- Mouse monoclonal [12F7] to 4 Hydroxynonenal
- Suitable for: WB
- Reacts with: Species independent
- Isotype: IgG1
Product nameAnti-4 Hydroxynonenal antibody [12F7]
See all 4 Hydroxynonenal primary antibodies
DescriptionMouse monoclonal [12F7] to 4 Hydroxynonenal
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Species independent
Chemical/ Small Molecule corresponding to 4 Hydroxynonenal conjugated to keyhole limpet haemocyanin.
- WB: 4 hydroxylnonenal-BSA.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein G purified
Purification notesPurified from TCS.
Our Abpromise guarantee covers the use of ab243070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
RelevanceAldehydic products of lipid peroxidation, such as 4 hydroxynonenal (4 HNE), have been implicated in the etiology of pathological changes under oxidative stress as a key mediator of oxidative stress induced cell death. It is a stable product of lipid peroxidation, is proarrhythmic and may contribute to the cytotoxic effects of oxidative stress.
- 4 HNE antibody
- 4-Hydroxy-2-Nonenal antibody
All lanes : Anti-4 Hydroxynonenal antibody [12F7] (ab243070) at 1/1000 dilution
Lane 1 : BSA at 0.5 µg
Lane 2 : 4 hydroxylnonenal-BSA at 0.5 µg
Lane 3 : 4 hydroxylnonenal-BSA at 2 µg
Lane 4 : 4-hydroxy-2-hexenal at 0.5 µg
Lane 5 : 4-hydroxy-2-hexenal at 2 µg
All lanes : Goat Anti-Mouse IgG: HRP at 1/2000 dilution
Developed using the ECL technique.
Exposure time: 5 minutes
Primary incubation for 2 hours at room temperature.
Secondary incubation for 1 hour at room temperature.
Block: 5% Skimmed milk in TBST.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab243070 has not yet been referenced specifically in any publications.