Overview

  • Product name

    Anti-4 Hydroxynonenal antibody [12F7]
    See all 4 Hydroxynonenal primary antibodies
  • Description

    Mouse monoclonal [12F7] to 4 Hydroxynonenal
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule corresponding to 4 Hydroxynonenal conjugated to keyhole limpet haemocyanin.

  • Positive control

    • WB: 4 hydroxylnonenal-BSA.

Properties

Applications

Our Abpromise guarantee covers the use of ab243070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.

Target

  • Relevance

    Aldehydic products of lipid peroxidation, such as 4 hydroxynonenal (4 HNE), have been implicated in the etiology of pathological changes under oxidative stress as a key mediator of oxidative stress induced cell death. It is a stable product of lipid peroxidation, is proarrhythmic and may contribute to the cytotoxic effects of oxidative stress.
  • Cellular localization

    Cytoplasmic
  • Alternative names

    • 4 HNE antibody
    • 4-Hydroxy-2-Nonenal antibody

Images

  • All lanes : Anti-4 Hydroxynonenal antibody [12F7] (ab243070) at 1/1000 dilution

    Lane 1 : BSA at 0.5 µg
    Lane 2 : 4 hydroxylnonenal-BSA at 0.5 µg
    Lane 3 : 4 hydroxylnonenal-BSA at 2 µg
    Lane 4 : 4-hydroxy-2-hexenal at 0.5 µg
    Lane 5 : 4-hydroxy-2-hexenal at 2 µg

    Secondary
    All lanes : Goat Anti-Mouse IgG: HRP at 1/2000 dilution

    Developed using the ECL technique.

    Exposure time: 5 minutes


    Primary incubation for 2 hours at room temperature.

    Secondary incubation for 1 hour at room temperature.

    Block: 5% Skimmed milk in TBST.

References

ab243070 has not yet been referenced specifically in any publications.

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