Product nameAnti-4 Hydroxynonenal antibody [12F7]
See all 4 Hydroxynonenal primary antibodies
DescriptionMouse monoclonal [12F7] to 4 Hydroxynonenal
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Species independent
Chemical/ Small Molecule corresponding to 4 Hydroxynonenal conjugated to keyhole limpet haemocyanin.
- WB: 4 hydroxylnonenal-BSA.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Purification notesPurified from TCS.
Our Abpromise guarantee covers the use of ab243070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
RelevanceAldehydic products of lipid peroxidation, such as 4 hydroxynonenal (4 HNE), have been implicated in the etiology of pathological changes under oxidative stress as a key mediator of oxidative stress induced cell death. It is a stable product of lipid peroxidation, is proarrhythmic and may contribute to the cytotoxic effects of oxidative stress.
- 4 HNE antibody
- 4-Hydroxy-2-Nonenal antibody
All lanes : Anti-4 Hydroxynonenal antibody [12F7] (ab243070) at 1/1000 dilution
Lane 1 : BSA at 0.5 µg
Lane 2 : 4 hydroxylnonenal-BSA at 0.5 µg
Lane 3 : 4 hydroxylnonenal-BSA at 2 µg
Lane 4 : 4-hydroxy-2-hexenal at 0.5 µg
Lane 5 : 4-hydroxy-2-hexenal at 2 µg
All lanes : Goat Anti-Mouse IgG: HRP at 1/2000 dilution
Developed using the ECL technique.
Exposure time: 5 minutes
Primary incubation for 2 hours at room temperature.
Secondary incubation for 1 hour at room temperature.
Block: 5% Skimmed milk in TBST.
ab243070 has not yet been referenced specifically in any publications.