Anti-4 Hydroxynonenal antibody [HNEJ-2] (ab48506)


  • Product name
    Anti-4 Hydroxynonenal antibody [HNEJ-2]
    See all 4 Hydroxynonenal primary antibodies
  • Description
    Mouse monoclonal [HNEJ-2] to 4 Hydroxynonenal
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ELISA, IHC-FoFrmore details
  • Immunogen

    Chemical/Small Molecule: 4-Hydroxy-2-Nonenal modified KLH.

  • Positive control
    • Colorectal carcinoma cells.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 28th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.



Our Abpromise guarantee covers the use of ab48506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 15 µg/ml.
IHC-P Use a concentration of 10 - 20 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. But NOTE: Fixation using Bouin's solution is recommended. In this case, antigen retrieval process is not required.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ELISA Use at an assay dependent concentration. PubMed: 22881716
IHC-FoFr Use at an assay dependent concentration.


  • Relevance
    Aldehydic products of lipid peroxidation, such as 4 hydroxynonenal (4 HNE), have been implicated in the etiology of pathological changes under oxidative stress as a key mediator of oxidative stress induced cell death. It is a stable product of lipid peroxidation, is proarrhythmic and may contribute to the cytotoxic effects of oxidative stress.
  • Cellular localization
  • Alternative names
    • 4 HNE antibody
    • 4-Hydroxy-2-Nonenal antibody


  • ab48506 at a 1/25 dilution staining 4-Hydroxy-2-Nonenal in mouse heart tissue sections by Immunohistochemistry (paraffin embedded) incubated for 15 hours at +4°C. Fixed with formaldehyde, heat mediated antigen retrieval step performed using citrate buffer. Blocked using 5% serum for 20 minutes at 20°C. Secondary used undiluted polyclonal Goat anti-mouse IgG conjugated to Alexa Fluor 594.

    See Abreview

  • ab15463 staining 4-Hydroxynonenal in the Mouse melanoma cancer tissue sections by IHC-FoFr (PFA perfusion fixed frozen Sections). Tissue samples were fixed with 4% Formalin, Antigen retrieval carried out heat mediation using anautoclave for 10 minutes, permeabilized using 0.1% SDS and blocked with 1% BSAfor 15 minutes at 25°C. The sample was incubated with primary antibody (1/25 in PBS + 1% BSA) at 4°C for 12 hours.An Alexa Fluor®488-conjugated Goat anti-mouse IgG(H+L) monoclonal(1/100) was used as the secondary antibody.

    See Abreview


This product has been referenced in:
  • Zhang X  et al. Reversible renal glomerular dysfunction in guinea pigs exposed to glutaraldehyde-polymerized cell-free hemoglobin. Toxicology 402-403:37-49 (2018). Read more (PubMed: 29689364) »
  • He Y  et al. Palmitic acid, but not high-glucose, induced myocardial apoptosis is alleviated by N-acetylcysteine due to attenuated mitochondrial-derived ROS accumulation-induced endoplasmic reticulum stress. Cell Death Dis 9:568 (2018). Read more (PubMed: 29752433) »

See all 53 Publications for this product

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (Liver)
Antigen retrieval step
Blocking step
Vector Lab M.O.M. blocking agent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.8% · Temperature: 25°C
Bouin's fixative

Abcam user community

Verified customer

Submitted Aug 19 2016

ab48506 antibody will detect only 4-HNE modified proteins.

This antibody is specific for 4-HNE-lysine, 4-HNE-histidine and
4-HNE-cysteine adduct.
By inhibition test, this antibody has a much higher affinity for the
4-HNE-histidine ...

Read More

Gracias por contactarnos.

No especificamos las especies para las que este anticuerpo está recomendado pues el antígeno corresponde a una estructura química, una molécula relativamente pequeña que es con...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (PFA perfusion fixed frozen sections)
Mouse Tissue sections (Melanoma cancer)
Melanoma cancer
4% Formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: autoclaved for 10min
Yes - 0.1% SDS
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 25°C

Ms. Seontae Kim

Verified customer

Submitted Dec 19 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Mouse Tissue lysate - whole (B16F10 melanoma)
Loading amount
30 µg
B16F10 melanoma
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Ms. Seontae Kim

Verified customer

Submitted Dec 18 2012

Thank you for contacting us.

For IHC-P we recommend to dilute the anti-4 Hydroxynonenal antibody [HNEJ-2] ab48506 in TBS with 1% BSA at a concentration of 10 - 20 µg/ml.
As indicated on the product datasheet, a heat mediated antigen ...

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Sorry, I neglectedadd that the mouse monoclonal, ab48506,has also been used in IHC in the following publications:

Li J et al. Up-regulation of p27kip1 contributes to Nrf2-mediated protection against angiotensin II-induced cardiac hypertrophy....

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Thank you for contacting us and your interest in our products.

The Anti-4 Hydroxynonenal antibody (ab46545) has been reported to work well for one of our customers in using the antibody in rat tissue sections. They found that using this antib...

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Thanks for sending along the document. I have forwarded it to our Accounts department. I will let you know what they say as soon as possible.

Please feel free tocontact me in the meantime if you have any other questions.

Thank you for contacting us and for the additional information.

I am sorry that this antibody did not perform as stated on the datasheet again, I have asked our Finance department to issue a credit note for you. The credit note may b...

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