Product nameAnti-4 Hydroxynonenal antibody [HNEJ-2]
See all 4 Hydroxynonenal primary antibodies
DescriptionMouse monoclonal [HNEJ-2] to 4 Hydroxynonenal
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ELISA, IHC-FoFrmore details
Chemical/Small Molecule: 4-Hydroxy-2-Nonenal modified KLH.
- Colorectal carcinoma cells.
This product was changed from ascites to tissue culture supernatant on 28th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituent: 0.41% PBS
PurityTissue culture supernatant
Light chain typekappa
Our Abpromise guarantee covers the use of ab48506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 - 15 µg/ml.|
|IHC-P||Use a concentration of 10 - 20 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. But NOTE: Fixation using Bouin's solution is recommended. In this case, antigen retrieval process is not required.|
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ELISA||Use at an assay dependent concentration. PubMed: 22881716|
|IHC-FoFr||Use at an assay dependent concentration.|
RelevanceAldehydic products of lipid peroxidation, such as 4 hydroxynonenal (4 HNE), have been implicated in the etiology of pathological changes under oxidative stress as a key mediator of oxidative stress induced cell death. It is a stable product of lipid peroxidation, is proarrhythmic and may contribute to the cytotoxic effects of oxidative stress.
- 4 HNE antibody
- 4-Hydroxy-2-Nonenal antibody
Elevated brain oxidative damage in ischemic 5xFAD mice
Formalin-fixed, paraffin-embedded mouse brain tissue (from non-trasngenic or 5xFAD transgenic animals) stained for 4 Hydroxynonenal using ab48506 at in immunohistochemical analysis.
Scale bar = 20μm. (A2) and (B2) are representative images of 4-HNE staining (Red) in brain cortex and CA1 region, respectively.
ab48506 at a 1/25 dilution staining 4-Hydroxy-2-Nonenal in mouse heart tissue sections by Immunohistochemistry (paraffin embedded) incubated for 15 hours at +4°C. Fixed with formaldehyde, heat mediated antigen retrieval step performed using citrate buffer. Blocked using 5% serum for 20 minutes at 20°C. Secondary used undiluted polyclonal Goat anti-mouse IgG conjugated to Alexa Fluor 594.
ab15463 staining 4-Hydroxynonenal in the Mouse melanoma cancer tissue sections by IHC-FoFr (PFA perfusion fixed frozen Sections). Tissue samples were fixed with 4% Formalin, Antigen retrieval carried out heat mediation using anautoclave for 10 minutes, permeabilized using 0.1% SDS and blocked with 1% BSAfor 15 minutes at 25°C. The sample was incubated with primary antibody (1/25 in PBS + 1% BSA) at 4°C for 12 hours.An Alexa Fluor®488-conjugated Goat anti-mouse IgG(H+L) monoclonal(1/100) was used as the secondary antibody.
This product has been referenced in:
- Kitaoka Y et al. Effects of Nrf2 deficiency on mitochondrial oxidative stress in aged skeletal muscle. Physiol Rep 7:e13998 (2019). Read more (PubMed: 30756520) »
- Jankovic A et al. Relation of Redox and Structural Alterations of Rat Skin in the Function of Chronological Aging. Oxid Med Cell Longev 2019:2471312 (2019). Read more (PubMed: 30906501) »