Product name4-Methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside, Fluorogenic glycanase substrate
DescriptionFluorogenic glycanase substrate.
Fluorogenic substrate for chitinases, chitotriosidases, peptidoglycan muramidase, and lysozyme. Can be used to screen plasma samples for reduced chitotriosidase activity that may be indicative of lysosomal storage disorders.
Fluorescence: λex 365 nm; λem 445 nm in 0.1 M phosphate pH 7.0 (lysozyme).
Storage instructionsStore at -20°C. Store In the Dark. Store under desiccating conditions. This product is air and light sensitive and impurities can occur as a result of air oxidation or due to metabolism by microbes.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
This product has been referenced in:
- Chang YM et al. Characterization of an acidic chitinase from seeds of black soybean (Glycine max (L) Merr Tainan No. 3). PLoS One 9:e113596 (2014). Read more (PubMed: 25437446) »
- Calderon C et al. Effect of human serum albumin on the kinetics of 4-methylumbelliferyl-ß-D-N-N'-N? Triacetylchitotrioside hydrolysis catalyzed by hen egg white lysozyme. Protein J 30:367-73 (2011). Read more (PubMed: 21748378) »
- Telkov MV et al. Proteins of the Rpf (resuscitation promoting factor) family are peptidoglycan hydrolases. Biochemistry (Mosc) 71:414-22 (2006). Read more (PubMed: 16615861) »
- Howard MB et al. Genomic analysis and initial characterization of the chitinolytic system of Microbulbifer degradans strain 2-40. J Bacteriol 185:3352-60 (2003). Read more (PubMed: 12754233) »