4-Methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside, Fluorogenic glycanase substrate (ab145023)

Overview

  • Product name

    4-Methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside, Fluorogenic glycanase substrate
  • Description

    Fluorogenic glycanase substrate.
  • Alternative names

    • 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside
    • 4-MU-GlcNAc3
    • GlcNAc1-b-4GlcNAc1-b-4GlcNAc1-b-4MU
    • MUF-triNAG
  • Biological description

    Fluorogenic substrate for chitinases, chitotriosidases, peptidoglycan muramidase, and lysozyme. Can be used to screen plasma samples for reduced chitotriosidase activity that may be indicative of lysosomal storage disorders.

  • General notes

    Fluorescence: λex 365 nm; λem 445 nm in 0.1 M phosphate pH 7.0 (lysozyme).

  • CAS Number

    53643-13-3
  • Chemical structure

    Chemical Structure

Properties

  • Excitation

    365nm
  • Emission

    445nm
  • Chemical name

    4-Methyl-7-coumarinyl-tri-N-acetyl-β-chitotrioside
  • Molecular weight

    785.75
  • Molecular formula

    C34H47N3O18
  • Storage instructions

    Store at -20°C. Store In the Dark. Store under desiccating conditions. This product is air and light sensitive and impurities can occur as a result of air oxidation or due to metabolism by microbes.
  • Solubility overview

    DMSO
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Synthetic

References

This product has been referenced in:

  • Chang YM  et al. Characterization of an acidic chitinase from seeds of black soybean (Glycine max (L) Merr Tainan No. 3). PLoS One 9:e113596 (2014). Read more (PubMed: 25437446) »
  • Calderon C  et al. Effect of human serum albumin on the kinetics of 4-methylumbelliferyl-ß-D-N-N'-N? Triacetylchitotrioside hydrolysis catalyzed by hen egg white lysozyme. Protein J 30:367-73 (2011). Read more (PubMed: 21748378) »
  • Telkov MV  et al. Proteins of the Rpf (resuscitation promoting factor) family are peptidoglycan hydrolases. Biochemistry (Mosc) 71:414-22 (2006). Read more (PubMed: 16615861) »
  • Howard MB  et al. Genomic analysis and initial characterization of the chitinolytic system of Microbulbifer degradans strain 2-40. J Bacteriol 185:3352-60 (2003). Read more (PubMed: 12754233) »

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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