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    450-nm-stop-solution-for-tmb-substrate-ab171529.pdf

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450 nm Stop Solution for TMB Substrate (ab171529)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (3)

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Overview

  • Product name

    450 nm Stop Solution for TMB Substrate
    See all ELISA stop solution reagents
  • Tested applications

    Suitable for: ELISAmore details
  • General notes

    Abcam’s 450 nm Stop Solution for TMB Substrate is a ready-to-use solution for colorimetric HRP-based ELISA detection. The acidic formulation is a proprietary solution used to stop TMB ELISA Substrate reactions (ab171522, ab171523, ab171524, ab171525, ab171526 and ab171527) and changes the chromogen from blue (620 nm) to yellow (450 nm) in color.

    Product Use:
    Equal volumes of TMB ELISA Substrate and 450 nm Stop Solution for TMB Substrate should be used. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at Room Temperature. Store at Room Temperature.
  • Storage buffer

    pH: 1
    Constituent: 5% Methanesulphonic acid
  • Concentration information loading...

Associated products

  • Related Products

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
    • 650 nm Stop Solution for TMB Substrate (ab171531)
    • Phosphate Buffered Saline (PBS) Casein ELISA Reagent (ab171532)
    • Tris Buffered Saline (TBS) Casein ELISA Reagent (ab171533)
    • Immunoassay Blocking Buffer (ab171534)
    • Immunoassay Blocking (BSA Free) (ab171535)
    • AP Stability Conjugate Reagent (ab171536)
    • HRP Stability Conjugate Reagent (ab171537)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab171529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration. Equal volumes of TMB ELISA Substrate and 450 nm Stop Solution for TMB Substrate should be used.
Notes
ELISA
Use at an assay dependent concentration. Equal volumes of TMB ELISA Substrate and 450 nm Stop Solution for TMB Substrate should be used.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (3)

    Publishing research using ab171529? Please let us know so that we can cite the reference in this datasheet.

    ab171529 has been referenced in 3 publications.

    • Martinez Viedma MDP  et al. Peptide arrays incubated with three collections of human sera from patients infected with mosquito-borne viruses. F1000Res 8:1875 (2019). PubMed: 32201571
    • Shen Z  et al. Delta-Like Ligand 4 Modulates Liver Damage by Down-Regulating Chemokine Expression. Am J Pathol 186:1874-1889 (2016). PubMed: 27171900
    • Hersey JS  et al. Functionalized Nanofiber Meshes Enhance Immunosorbent Assays. Anal Chem 87:11863-70 (2015). PubMed: 26551162

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    How long is the reacted product stable following the use of ab171529?

    Read More

    Abcam community

    Verified customer

    Asked on May 14 2013

    Answer

    At least one hour.

    Read More

    Abcam Scientific Support

    Answered on May 14 2013

    Question

    Why are 450 nm Stop Solution for TMB Substrates recommended for stopping TMB substrates?

    Read More

    Abcam community

    Verified customer

    Asked on May 14 2013

    Answer

    They have greater signal stability at the high end of the standard curve, which increases dynamic range and reproducibility.

    Read More

    Abcam Scientific Support

    Answered on May 14 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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