450 nm Stop Solution for TMB Substrate (ab171529)
- Datasheet
- References (2)
- Protocols
Overview
-
Product name
450 nm Stop Solution for TMB Substrate
See all ELISA stop solution reagents -
Tested applications
Suitable for: ELISAmore details -
General notes
Abcam’s 450 nm Stop Solution for TMB Substrate is a ready-to-use solution for colorimetric HRP-based ELISA detection. The acidic formulation is a proprietary solution used to stop TMB ELISA Substrate reactions (ab171522, ab171523, ab171524, ab171525, ab171526 and ab171527) and changes the chromogen from blue (620 nm) to yellow (450 nm) in color.
Product Use:
Equal volumes of TMB ELISA Substrate and 450 nm Stop Solution for TMB Substrate should be used.Storage Instructions:
ab171529 should be stored at 2-8°C. Exposure to direct sunlight and other UV sources should be avoided due to the light sensitive nature of the TMB molecule.
Properties
-
Form
Liquid -
Storage instructions
Store at +4°C. It is important to note that this product is reported to be light sensitive. Please see notes section. -
Storage buffer
Constituent: 5% Methanesulphonic acid -
Concentration information loading...
Associated products
-
Related Products
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Phosphate Buffered Saline (PBS) Casein ELISA Reagent (ab171532)
- Tris Buffered Saline (TBS) Casein ELISA Reagent (ab171533)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
- AP Stability Conjugate Reagent (ab171536)
- HRP Stability Conjugate Reagent (ab171537)
Applications
Our Abpromise guarantee covers the use of ab171529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. Equal volumes of TMB ELISA Substrate and 450 nm Stop Solution for TMB Substrate should be used. |
References
This product has been referenced in:
- Shen Z et al. Delta-Like Ligand 4 Modulates Liver Damage by Down-Regulating Chemokine Expression. Am J Pathol 186:1874-1889 (2016). Read more (PubMed: 27171900) »
- Hersey JS et al. Functionalized Nanofiber Meshes Enhance Immunosorbent Assays. Anal Chem 87:11863-70 (2015). Read more (PubMed: 26551162) »
