450 nm Stop Solution for TMB Substrate (ab171529)
Overview
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Product name
450 nm Stop Solution for TMB Substrate
See all ELISA stop solution reagents -
Tested applications
Suitable for: ELISAmore details -
General notes
Abcam’s 450 nm Stop Solution for TMB Substrate is a ready-to-use solution for colorimetric HRP-based ELISA detection. The acidic formulation is a proprietary solution used to stop TMB ELISA Substrate reactions (ab171522, ab171523, ab171524, ab171525, ab171526 and ab171527) and changes the chromogen from blue (620 nm) to yellow (450 nm) in color.
Product Use:
Equal volumes of TMB ELISA Substrate and 450 nm Stop Solution for TMB Substrate should be used.
Properties
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Form
Liquid -
Storage instructions
Shipped at Room Temperature. Store at Room Temperature. -
Storage buffer
pH: 1
Constituent: 5% Methanesulphonic acid -
Concentration information loading...
Associated products
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Related Products
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Phosphate Buffered Saline (PBS) Casein ELISA Reagent (ab171532)
- Tris Buffered Saline (TBS) Casein ELISA Reagent (ab171533)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
- AP Stability Conjugate Reagent (ab171536)
- HRP Stability Conjugate Reagent (ab171537)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab171529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration. Equal volumes of TMB ELISA Substrate and 450 nm Stop Solution for TMB Substrate should be used.
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| Notes |
|---|
|
ELISA
Use at an assay dependent concentration. Equal volumes of TMB ELISA Substrate and 450 nm Stop Solution for TMB Substrate should be used. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab171529 has been referenced in 6 publications.
- Camacho-Sandoval R et al. Development and Evaluation of a Set of Spike and Receptor Binding Domain-Based Enzyme-Linked Immunosorbent Assays for SARS-CoV-2 Serological Testing. Diagnostics (Basel) 11:N/A (2021). PubMed: 34441440
- Gaudreault NN et al. SARS-CoV-2 infection, disease and transmission in domestic cats. Emerg Microbes Infect 9:2322-2332 (2020). PubMed: 33028154
- Harland M et al. Neuronal Mitochondria Modulation of LPS-Induced Neuroinflammation. J Neurosci 40:1756-1765 (2020). PubMed: 31937559
- Martinez Viedma MDP et al. Peptide arrays incubated with three collections of human sera from patients infected with mosquito-borne viruses. F1000Res 8:1875 (2019). PubMed: 32201571
- Shen Z et al. Delta-Like Ligand 4 Modulates Liver Damage by Down-Regulating Chemokine Expression. Am J Pathol 186:1874-1889 (2016). PubMed: 27171900
- Hersey JS et al. Functionalized Nanofiber Meshes Enhance Immunosorbent Assays. Anal Chem 87:11863-70 (2015). PubMed: 26551162