For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    450-nm-stop-solution-for-tmb-substrate-ab171529.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Share by email

450 nm Stop Solution for TMB Substrate (ab171529)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (6)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

You may also be interested in

Primary
Product image
Anti-Alpha-synuclein (phospho Y125) antibody [EPR2179(2)] (ab124955)
Primary
Product image
Anti-EGFR antibody [EP38Y] - Low endotoxin, Azide free (ab174481)
Primary
Product image
Anti-Prostate Specific Antigen antibody [EP1588Y] (ab76113)

View more associated products

Overview

  • Product name

    450 nm Stop Solution for TMB Substrate
    See all ELISA stop solution reagents
  • Tested applications

    Suitable for: ELISAmore details
  • General notes

    Abcam’s 450 nm Stop Solution for TMB Substrate is a ready-to-use solution for colorimetric HRP-based ELISA detection. The acidic formulation is a proprietary solution used to stop TMB ELISA Substrate reactions (ab171522, ab171523, ab171524, ab171525, ab171526 and ab171527) and changes the chromogen from blue (620 nm) to yellow (450 nm) in color.

    Product Use:
    Equal volumes of TMB ELISA Substrate and 450 nm Stop Solution for TMB Substrate should be used. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at Room Temperature. Store at Room Temperature.
  • Storage buffer

    pH: 1
    Constituent: 5% Methanesulphonic acid
  • Concentration information loading...

Associated products

  • Related Products

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
    • 650 nm Stop Solution for TMB Substrate (ab171531)
    • Phosphate Buffered Saline (PBS) Casein ELISA Reagent (ab171532)
    • Tris Buffered Saline (TBS) Casein ELISA Reagent (ab171533)
    • Immunoassay Blocking Buffer (ab171534)
    • Immunoassay Blocking (BSA Free) (ab171535)
    • AP Stability Conjugate Reagent (ab171536)
    • HRP Stability Conjugate Reagent (ab171537)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab171529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration. Equal volumes of TMB ELISA Substrate and 450 nm Stop Solution for TMB Substrate should be used.
Notes
ELISA
Use at an assay dependent concentration. Equal volumes of TMB ELISA Substrate and 450 nm Stop Solution for TMB Substrate should be used.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (6)

Publishing research using ab171529? Please let us know so that we can cite the reference in this datasheet.

ab171529 has been referenced in 6 publications.

  • Camacho-Sandoval R  et al. Development and Evaluation of a Set of Spike and Receptor Binding Domain-Based Enzyme-Linked Immunosorbent Assays for SARS-CoV-2 Serological Testing. Diagnostics (Basel) 11:N/A (2021). PubMed: 34441440
  • Gaudreault NN  et al. SARS-CoV-2 infection, disease and transmission in domestic cats. Emerg Microbes Infect 9:2322-2332 (2020). PubMed: 33028154
  • Harland M  et al. Neuronal Mitochondria Modulation of LPS-Induced Neuroinflammation. J Neurosci 40:1756-1765 (2020). PubMed: 31937559
  • Martinez Viedma MDP  et al. Peptide arrays incubated with three collections of human sera from patients infected with mosquito-borne viruses. F1000Res 8:1875 (2019). PubMed: 32201571
  • Shen Z  et al. Delta-Like Ligand 4 Modulates Liver Damage by Down-Regulating Chemokine Expression. Am J Pathol 186:1874-1889 (2016). PubMed: 27171900
  • Hersey JS  et al. Functionalized Nanofiber Meshes Enhance Immunosorbent Assays. Anal Chem 87:11863-70 (2015). PubMed: 26551162

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Question

How long is the reacted product stable following the use of ab171529?

Read More

Abcam community

Verified customer

Asked on May 14 2013

Answer

At least one hour.

Read More

Abcam Scientific Support

Answered on May 14 2013

Question

Why are 450 nm Stop Solution for TMB Substrates recommended for stopping TMB substrates?

Read More

Abcam community

Verified customer

Asked on May 14 2013

Answer

They have greater signal stability at the high end of the standard curve, which increases dynamic range and reproducibility.

Read More

Abcam Scientific Support

Answered on May 14 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.