Product nameAnti-4E-T antibody
See all 4E-T primary antibodies
DescriptionRabbit polyclonal to 4E-T
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus
Synthetic peptide corresponding to Human 4E-T aa 925-985.
- HeLa whole cell lysate, 293T whole cell lysate and mouse NIH3T3 whole cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab95030 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 108 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionNucleoplasmic shuttling protein. Mediates the nuclear import of EIF4E by a piggy-back mechanism.
Tissue specificityWidely expressed.
Cellular localizationCytoplasm. Nucleus. Predominantly cytoplasmic. Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner.
- Information by UniProt
- 2610509L04Rik antibody
- 4E T antibody
- 4E-T antibody
All lanes : Anti-4E-T antibody (ab95030) at 1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 108 kDa
Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling 4E-T with ab95030 at 1/1000 (1µg/ml). Detection: DAB.
Detection of 4E-T in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab95030 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab95030 has not yet been referenced specifically in any publications.