Anti-5 Lipoxygenase/5-LO (phospho S523) antibody (ab30573)
Key features and details
- Rabbit polyclonal to 5 Lipoxygenase/5-LO (phospho S523)
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-5 Lipoxygenase/5-LO (phospho S523) antibody
See all 5 Lipoxygenase/5-LO primary antibodies -
Description
Rabbit polyclonal to 5 Lipoxygenase/5-LO (phospho S523) -
Host species
Rabbit -
Specificity
Specific for the ~80k 5 Lipoxygenase/5-LO phosphorylated at Ser523 in western blots of rat hippocampal lysates.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Synthetic peptide within Human 5 Lipoxygenase/5-LO (phospho S523). The exact sequence is proprietary.
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Positive control
- WB: Rat cortex tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.50
Constituents: 0.238% HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab30573 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 78 kDa).
The immunolabeling is completely eliminated by treatment with lambda-Phosphatase. |
Notes |
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WB
1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 78 kDa). The immunolabeling is completely eliminated by treatment with lambda-Phosphatase. |
Target
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Function
Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes. -
Pathway
Lipid metabolism; leukotriene A4 biosynthesis. -
Sequence similarities
Belongs to the lipoxygenase family.
Contains 1 lipoxygenase domain.
Contains 1 PLAT domain. -
Post-translational
modificationsSerine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus. -
Cellular localization
Cytoplasm. Nucleus matrix. Nucleus membrane. Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association. - Information by UniProt
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Database links
- Entrez Gene: 25290 Rat
- SwissProt: P12527 Rat
- Unigene: 9662 Rat
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Alternative names
- 5 Lipoxygenase antibody
- 5 LO antibody
- 5 LOX antibody
see all
Images
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All lanes : Anti-5 Lipoxygenase/5-LO (phospho S523) antibody (ab30573) at 1/1000 dilution
All lanes : rat cortex lysate
Predicted band size: 78 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Lane 2 was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser523 5Lipoxygenase
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab30573 has been referenced in 1 publication.
- Sun J et al. Anti-allergic actions of a Chinese patent medicine, huoxiangzhengqi oral liquid, in RBL-2H3 cells and in mice. Pharm Biol 59:672-682 (2021). PubMed: 34078224