Product name5-Bromo-4-chloro-3-indolyl beta-D-glucuronide cyclohexylammonium salt
DescriptionChromogenic β-glucuronidase substrate.
- 5-bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt
- X-GlcU CHA
Chromogenic substrate for β-glucuronidase that is converted by the enzyme into an intense blue prepicitate. Used for the detection of the Gus gene in bacterial colonies.
Chemical name5-Bromo-4-chloro-1H-indol-3-yl β-D-glucopyranosiduronic acid cyclohexylammonium salt
Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewDMSO, water
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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This product has been referenced in:
- Kodaka H et al. Evaluation of new medium with chromogenic substrates for members of the family Enterobacteriaceae in urine samples. J Clin Microbiol 33:199-201 (1995). Read more (PubMed: 7699041) »
- Jefferson RA et al. beta-Glucuronidase from Escherichia coli as a gene-fusion marker. Proc Natl Acad Sci U S A 83:8447-51 (1986). Read more (PubMed: 3534890) »