Key features and details
- Rabbit polyclonal to 5-carboxylcytosine (5-caC)
- Suitable for: IP, Dot blot, ICC/IF
- Reacts with: Species independent
- Isotype: IgG
Product nameAnti-5-carboxylcytosine (5-caC) antibody
DescriptionRabbit polyclonal to 5-carboxylcytosine (5-caC)
Tested applicationsSuitable for: IP, Dot blot, ICC/IFmore details
Species reactivityReacts with: Species independent
Chemical/ Small Molecule corresponding to 5-carboxylcytosine (5-caC) conjugated to bovine serum albumin.
- ICC/IF: HEK-293T cells transfected with mouse FLAG-tagged wild-type Tet1 (Tet1 CD).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 30% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab231801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
4 µg per IP.
|Dot blot||1/500 - 1/1000.|
- 5 c C antibody
- 5 ca C antibody
HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either the mouse FLAG-tagged wild-type Tet1 (Tet1 CD) or the catalytically inactive FLAG-tagged C-terminal domain of Tet1 (Tet1 mCD)stained for 5-carboxylcytosine (5-caC) using ab231801 at a dilution of 1/500 in ICC/IF.
Immunoprecipitation was performed with ab231801 on 2 μg of J1 ES genomic DNA, spiked with 1 pg of a control DNA fragment (approximately 700 bp from the RFP (Ring fnger protein) gene) containing different cytosine modifcations.
The mC and hmC control DNA was generated by PCR with the corresponding nucleotide. The caC control fragment was obtained by in vitro methylation using M.SssI methyltransferase followed by oxidation with purifed Tet2. The IP’d DNA was subsequently anaysed by qPCR using primers specifc for the control DNA fragments and for GAPDH, used as a negative control. Image shows the enrichment calculated as the ratio of the recovery of the control DNA versus the recovery of the GAPDH negative control.
To demonstrate the specifcity of ab231801 a Dot Blot analysis was performed using synthetic oligonucleotides containing different modifed C-bases (indicated in red).
125 and 25 ng of the respective oligo’s were bound to a Streptavindin-coated multi-well plate. ab231801 was used at a dilution of 1:1,000. The binding of antibody to the DNA was measured by ECL chemiluminescence.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231801 has not yet been referenced specifically in any publications.