5-CFDA (5-Carboxyfluorescein Diacetate), Fluorogenic non-selective esterase substrate (ab145256)

Overview

  • Product name

    5-CFDA (5-Carboxyfluorescein Diacetate), Fluorogenic non-selective esterase substrate
  • Description

    Hydrophobic, cell-permeable fluorogenic non-selective esterase substrate
  • Biological description

    Hydrophobic, cell-permeable fluorogenic non-selective esterase substrate. Becomes hydrolyzed to hydrophilic strongly fluorescent 5-Carboxyfluorescein (ab145293) intracellularly. Measures intracellular pH. 5-CFDA N-succinimidyl ester (ab145292) precursor.

  • Purity

    > 95%
  • CAS Number

    79955-27-4
  • Chemical structure

    Chemical Structure

Properties

  • Excitation

    495nm
  • Emission

    520nm
  • Chemical name

    3',6'-Diacetyloxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid
  • Molecular weight

    460.39
  • Molecular formula

    C25H16O9
  • PubChem identifier

    133314
  • Storage instructions

    Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in ethanol to 10 mM and in DMSO
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    CC(=O)OC1=CC2=C(C=C1)C3(C4=C(C=C(C=C4)C(=O)O)C(=O)O3)C5=C(O2)C=C(C=C5)OC(=O)C
  • Source

    Synthetic

  • Research areas

References

This product has been referenced in:

  • Gorokhova E  et al. A comparison of TO-PRO-1 iodide and 5-CFDA-AM staining methods for assessing viability of planktonic algae with epifluorescence microscopy. J Microbiol Methods 89:216-21 (2012). Read more (PubMed: 22446098) »
  • Schreer A  et al. Application of Alamar blue/5-carboxyfluorescein diacetate acetoxymethyl ester as a noninvasive cell viability assay in primary hepatocytes from rainbow trout. Anal Biochem 344:76-85 (2005). Read more (PubMed: 16039980) »
  • Fuller ME  et al. Development of a vital fluorescent staining method for monitoring bacterial transport in subsurface environments. Appl Environ Microbiol 66:4486-96 (2000). Read more (PubMed: 11010903) »

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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