Product name5-Ethynyl-2'-deoxycytidine (5-EdC), DNA synthesis monitoring probe
DescriptionDNA synthesis monitoring probe
- Ethynyl-labeled deoxycytidine
Relatively nontoxic 2’-deoxycytidine analog. Alternative to BrdU (5-Bromo-2’-deoxyuridine) (ab142567) for measuring de novo DNA synthesis during S-phase. Incorporates into replicating DNA instead of its natural analog thymidine.
Storage instructionsStore at -20°C. Store In the Dark. Store under desiccating conditions. This product is air and light sensitive and impurities can occur as a result of air oxidation or due to metabolism by microbes.
Solubility overviewSoluble in DMSO
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
This product has been referenced in:
- Ligasová A et al. Dr Jekyll and Mr Hyde: a strange case of 5-ethynyl-2'-deoxyuridine and 5-ethynyl-2'-deoxycytidine. Open Biol 6:150172 (2016). Read more (PubMed: 26740587) »
- Qu D et al. 5-Ethynyl-2'-deoxycytidine as a new agent for DNA labeling: detection of proliferating cells. Anal Biochem 417:112-21 (2011). Read more (PubMed: 21683679) »
- Guan L et al. Intracellular detection of cytosine incorporation in genomic DNA by using 5-ethynyl-2'-deoxycytidine. Chembiochem 12:2184-90 (2011). Read more (PubMed: 21805552) »