Key features and details
- Rabbit polyclonal to 5-formylcytosine (5-fC)
- Suitable for: IP
- Isotype: IgG
Product nameAnti-5-formylcytosine (5-fC) antibody
DescriptionRabbit polyclonal to 5-formylcytosine (5-fC)
Tested applicationsSuitable for: IPmore details
Chemical/ Small Molecule corresponding to 5-formylcytosine (5-fC) conjugated to keyhole limpet haemocyanin.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab231898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
3 µl per IP reaction.
- 5 f C antibody
- 5 fC antibody
- 5 formyl cytosine antibody
- 5fC antibody
HEK-293 (Human epithelial cell line from embryonic kidney) cells were transfected with a reporter gene and hydroxymethylated in vitro with either a pCAG expression vector containing the TET2 catalytic domain (TET2cd) or a negative control pCAG vector.
DIP assays were performed on 4 µg of sheared and denatured DNA using 3 µl of ab231898 in a total of 500 µl IP buffer. QPCR was performed with primers specifc for the reporter gene. Image shows the recovery, expressed as a % of input (mean +standard deviation of 3 different experiments).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231898 has not yet been referenced specifically in any publications.