Product nameAnti-5-formylcytosine (5-fC) antibody
DescriptionRabbit polyclonal to 5-formylcytosine (5-fC)
Tested applicationsSuitable for: IPmore details
Chemical/ Small Molecule corresponding to 5-formylcytosine (5-fC) conjugated to keyhole limpet haemocyanin.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Our Abpromise guarantee covers the use of ab231898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
3 µl per IP reaction.
- 5 f C antibody
- 5 fC antibody
- 5 formyl cytosine antibody
- 5fC antibody
HEK-293 (Human epithelial cell line from embryonic kidney) cells were transfected with a reporter gene and hydroxymethylated in vitro with either a pCAG expression vector containing the TET2 catalytic domain (TET2cd) or a negative control pCAG vector.
DIP assays were performed on 4 µg of sheared and denatured DNA using 3 µl of ab231898 in a total of 500 µl IP buffer. QPCR was performed with primers specifc for the reporter gene. Image shows the recovery, expressed as a % of input (mean +standard deviation of 3 different experiments).
ab231898 has not yet been referenced specifically in any publications.