Overview

  • Product name

    Anti-5-formylcytosine (5-fC) antibody
  • Description

    Rabbit polyclonal to 5-formylcytosine (5-fC)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
  • Immunogen

    Chemical/ Small Molecule corresponding to 5-formylcytosine (5-fC) conjugated to keyhole limpet haemocyanin.

Properties

Applications

Our Abpromise guarantee covers the use of ab231898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.

3 µl per IP reaction.

Target

  • Alternative names

    • 5 f C antibody
    • 5 fC antibody
    • 5 formyl cytosine antibody
    • 5fC antibody

Images

  • HEK-293 (Human epithelial cell line from embryonic kidney) cells were transfected with a reporter gene and hydroxymethylated in vitro with either a pCAG expression vector containing the TET2 catalytic domain (TET2cd) or a negative control pCAG vector.

    DIP assays were performed on 4 µg of sheared and denatured DNA using 3 µl of ab231898 in a total of 500 µl IP buffer. QPCR was performed with primers specifc for the reporter gene. Image shows the recovery, expressed as a % of input (mean +standard deviation of 3 different experiments).

References

ab231898 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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