Anti-5-HT2C Receptor antibody (ab137529)
Key features and details
- Rabbit polyclonal to 5-HT2C Receptor
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-5-HT2C Receptor antibody
See all 5-HT2C Receptor primary antibodies -
Description
Rabbit polyclonal to 5-HT2C Receptor -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Dog
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Immunogen
Synthetic peptide within Human 5HT2C Receptor aa 387-436. The exact sequence is proprietary.
Database link: P28335 -
Positive control
- WB: A431, rat brain tissue, IMR32, SK-N-AS cell extracts. IHC-P: Human prostate tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
- HeLa whole cell lysate (ab150035)
- HeLa whole cell lysate (ab29545)
- HeLa membrane extract lysate (ab29547)
- Raji whole cell lysate (ab30124)
- Raji membrane extract lysate (ab30126)
- A-431 whole cell lysate (ab30132)
- A-431 membrane extract lysate (ab30134)
- A-431 whole cell lysate (ab7909)
- MOLT-4 whole cell lysate (ab7912)
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 52 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 52 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
Target
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Function
This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Domain
The PDZ domain-binding motif is involved in the interaction with MPDZ. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 450240 Dog
- Entrez Gene: 3358 Human
- Entrez Gene: 15560 Mouse
- Entrez Gene: 25187 Rat
- Omim: 312861 Human
- SwissProt: Q60F97 Dog
- SwissProt: P28335 Human
- SwissProt: P34968 Mouse
see all -
Alternative names
- 5 Hydroxytryptamine 2C receptor antibody
- 5-HT-1C antibody
- 5-ht-1c receptor antibody
see all
Images
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Western blot - Anti-5-HT2C Receptor antibody (ab137529)Anti-5-HT2C Receptor antibody (ab137529) at 1/1000 dilution + Rat brain tissue extract at 50 µg
Predicted band size: 52 kDa10% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5-HT2C Receptor antibody (ab137529)Immunohistochemical analysis of paraffin embedded Human prostate tissue labelling 5HT2C Receptor with ab137529 at 10 µg/ml. -
Western blot - Anti-5-HT2C Receptor antibody (ab137529)All lanes : Anti-5-HT2C Receptor antibody (ab137529) at 1/500 dilution
Lane 1 : IMR32 whole cell extract
Lane 2 : SK-N-AS whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 52 kDa10% SDS-PAGE
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Western blot - Anti-5-HT2C Receptor antibody (ab137529)Anti-5-HT2C Receptor antibody (ab137529) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 52 kDa
10% SDS PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab137529 has been referenced in 3 publications.
- Wang Z et al. Basolateral Amygdala Serotonin 2C Receptor Regulates Emotional Disorder-Related Symptoms Induced by Chronic Methamphetamine Administration. Front Pharmacol 12:627307 (2021). PubMed: 33628192
- Zhu H et al. Long-term stability and characteristics of behavioral, biochemical, and molecular markers of three different rodent models for depression. Brain Behav 10:e01508 (2020). PubMed: 31867894
- Baptista-de-Souza D et al. Chronic Fluoxetine Impairs the Effects of 5-HT1A and 5-HT2C Receptors Activation in the PAG and Amygdala on Antinociception Induced by Aversive Situation in Mice. Front Pharmacol 11:260 (2020). PubMed: 32218734