Recombinant Anti-5-HT2C Receptor antibody [EPR6487] (ab133570)
![Western blot - Anti-5-HT2C Receptor antibody [EPR6487] (ab133570)](https://www.abcam.com/ps/products/133/ab133570/Images/ab133570-327941-anti-5ht2c-receptor-antibody-epr6487-western-blot.jpg "Western blot - Anti-5-HT2C Receptor antibody [EPR6487] (ab133570)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6487] to 5-HT2C Receptor
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-5-HT2C Receptor antibody [EPR6487]
See all 5-HT2C Receptor primary antibodies -
Description
Rabbit monoclonal [EPR6487] to 5-HT2C Receptor -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
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Positive control
- WB: SH-SY5Y whole cell lysate and human brain tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 9.40 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6487 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133570 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 52 kDa. |
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP.
Target
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Function
This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Domain
The PDZ domain-binding motif is involved in the interaction with MPDZ. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 3358 Human
- Omim: 312861 Human
- SwissProt: P28335 Human
- Unigene: 149037 Human
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Alternative names
- 5 Hydroxytryptamine 2C receptor antibody
- 5-HT-1C antibody
- 5-ht-1c receptor antibody
see all
Images
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Western blot - Anti-5-HT2C Receptor antibody [EPR6487] (ab133570)All lanes : Anti-5-HT2C Receptor antibody [EPR6487] (ab133570) at 1/1000 dilution
Lane 1 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates
Lane 2 : Human brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Diluting buffer and concentration 5% NFDM/TBST
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![OI-RD Scanning - Anti-5-HT2C Receptor antibody [EPR6487] (ab133570)](https://www.abcam.com/ps/products/133/ab133570/Images/ab133570-147342-anti-5ht2c-receptor-antibody-epr6487.jpg)
OI-RD Scanning - Anti-5-HT2C Receptor antibody [EPR6487] (ab133570) -
![Anti-5-HT2C Receptor antibody [EPR6487] (ab133570)](https://www.abcam.com/ps/products/133/ab133570/Images/ab133570-5-benefits-of-recombinant-antibodies.png)
Anti-5-HT2C Receptor antibody [EPR6487] (ab133570)
Datasheets and documents
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Datasheet download
References (5)
ab133570 has been referenced in 5 publications.
- Xue Y et al. Down-regulation of Spinal 5-HT2A and 5-HT2C Receptors Contributes to Somatic Hyperalgesia induced by Orofacial Inflammation Combined with Stress. Neuroscience 440:196-209 (2020). PubMed: 32497757
- Pellegrini L et al. SARS-CoV-2 Infects the Brain Choroid Plexus and Disrupts the Blood-CSF Barrier in Human Brain Organoids. Cell Stem Cell 27:951-961.e5 (2020). PubMed: 33113348
- Xu GZ et al. Valproate reverses stress-induced somatic hyperalgesia and visceral hypersensitivity by up-regulating spinal 5-HT2C receptor expression in female rats. Neuropharmacology 165:107926 (2020). PubMed: 31883927
- Nyarko JNK et al. Glycosylation States of Pre- and Post-synaptic Markers of 5-HT Neurons Differ With Sex and 5-HTTLPR Genotype in Cortical Autopsy Samples. Front Neurosci 12:545 (2018). PubMed: 30147642
- Ji G et al. 5-HT2CReceptor Knockdown in the Amygdala Inhibits Neuropathic-Pain-Related Plasticity and Behaviors. J Neurosci 37:1378-1393 (2017). PubMed: 28011743
