Overview

  • Product name

    Anti-5-Hydroxymethyl-2’-deoxyuridine antibody
  • Description

    Goat polyclonal to 5-Hydroxymethyl-2’-deoxyuridine
  • Host species

    Goat
  • Tested applications

    Suitable for: ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    5-Hydroxymethyl-2’-deoxyuridine conjugate

  • Positive control

    • Alzheimer brain homogenates

Properties

Applications

Our Abpromise guarantee covers the use of ab95259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Target

  • Relevance

    5-Hydroxymethyl-2’-deoxyuridine (5-HmdU) is formed from thymidine during the oxidation of DNA by ionizing radiation or reactive oxygen species (ROS) that are formed from inflammatory response. This causes genetic mutations, altered gene expression and chromosomal instability that have generally been implicated with aging, cancers and autoimmune diseases. Systemic oxidative stress levels, as evidenced by DNA damage products in the blood and urine, appear to be associated with a risk of various cancers, including that of the breast, lung and colorectal cancers. Cigarette smoke contains numerous chemical carcinogens and other compounds that generate reactive oxygen species that can damage DNA directly or indirectly via inflammatory processes. 5-HmdU is one of the major oxidized DNA bases that is significantly elevated in the white blood cells of women diagnosed with breast cancer, which may be a result of diminished DNA repair. Individuals with BRCA1 and BRCA2 mutations may be at increased risk for cancer due to deficiencies in the repair of DNA lesions caused by ROS. Additionally, antibody titers recognizing 5-HmdU have also been found to be significantly elevated in women with breast, color and rectal cancers. Therefore, oxidative DNA damage is therefore an attractive marker of disease risk as it takes into account not only the exposure to and production of oxidants, but also the cells’ DNA repair ability.

References

ab95259 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number XXXXXX. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

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Answer

Thank you for contacting us.


While Dot Blot does indeed have a lot of similarity to a non-reduced/denatured western blot or with an indirect ELISA, we do consider it to be a separate application from either of these. We do however recognize that many products have not been tested for use in this application and as such using a product which is guaranteed for use in one of these is probably the best bet when searching for antibodies.

If you could provide me with the details of your protocol and with any troubleshooting steps and images which you may have, I would be very happy to review these and see if I might be able to provide suggestions to improve the performance of these products.While these are not guaranteed for use in Dot Blot, should we not be able to provide help with these I am sure we might be able to find a way to come to a result which would be satisfactory to both Abcam and you.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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