Key features and details
- Rabbit polyclonal to 5-hydroxymethylcytosine (5-hmC)
- Suitable for: IP, Dot blot
- Isotype: IgG
Product nameAnti-5-hydroxymethylcytosine (5-hmC) antibody
See all 5-hydroxymethylcytosine (5-hmC) primary antibodies
DescriptionRabbit polyclonal to 5-hydroxymethylcytosine (5-hmC)
Tested applicationsSuitable for: IP, Dot blotmore details
Chemical/ Small Molecule corresponding to 5-hydroxymethylcytosine (5-hmC) conjugated to keyhole limpet haemocyanin.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab231902 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
Relevance5-Hydroxymethylcytosine (5-hmC) is a modified base form of cytosine recently found in Human/mouse brain and inembryonic stem cells. This DNA pyrimidine nitrogen base can be generated by oxidation of 5-methylcytosine, a reaction mediated by the ten-eleven translocation (TET) family of the 5-mC hydroxylases. The function of this base is still not elucidated but it is believed to play an important role in switching genes on and off.
- 5-hmC antibody
An hydroxymethylated DNA IP (hMeDIP) was performed using ab231902.
IgG isotype antibodies from rabbit were used as negative controls. DNA fragments of 300-500 bp were used. 1 µg of Hela (Human epithelial cell line from cervix adenocarcinoma) cells DNA were spiked with non-methylated, methylated, and hydroxymethylated fragments. The IP’d material has been analysed by qPCR using the primer pair specifc for the 3 different control sequences.
Lane 1: Unmethylated control.
Lane 2: Methylated control.
Lane 3: Hydroxymethylated control.
Dot Blot analysis of ab231902 with the C, mC and hmC PCR controls.
100 to 4 ng (equivalent of 5 to 0.2 pmol of C-bases) of the hmC, mC and C PCR controls were spotted on a membrane, then incubated with ab231902 at a 1:200 dilution.
Exposure time: 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231902 has not yet been referenced specifically in any publications.