Product nameAnti-5-hydroxymethylcytosine (5-hmC) antibody
See all 5-hydroxymethylcytosine (5-hmC) primary antibodies
DescriptionRabbit polyclonal to 5-hydroxymethylcytosine (5-hmC)
Tested applicationsSuitable for: IP, Dot blotmore details
Chemical/ Small Molecule corresponding to 5-hydroxymethylcytosine (5-hmC) conjugated to keyhole limpet haemocyanin.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Our Abpromise guarantee covers the use of ab231902 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
Relevance5-Hydroxymethylcytosine (5-hmC) is a modified base form of cytosine recently found in Human/mouse brain and inembryonic stem cells. This DNA pyrimidine nitrogen base can be generated by oxidation of 5-methylcytosine, a reaction mediated by the ten-eleven translocation (TET) family of the 5-mC hydroxylases. The function of this base is still not elucidated but it is believed to play an important role in switching genes on and off.
- 5-hmC antibody
An hydroxymethylated DNA IP (hMeDIP) was performed using ab231902.
IgG isotype antibodies from rabbit were used as negative controls. DNA fragments of 300-500 bp were used. 1 µg of Hela (Human epithelial cell line from cervix adenocarcinoma) cells DNA were spiked with non-methylated, methylated, and hydroxymethylated fragments. The IP’d material has been analysed by qPCR using the primer pair specifc for the 3 different control sequences.
Lane 1: Unmethylated control.
Lane 2: Methylated control.
Lane 3: Hydroxymethylated control.
Dot Blot analysis of ab231902 with the C, mC and hmC PCR controls.
100 to 4 ng (equivalent of 5 to 0.2 pmol of C-bases) of the hmC, mC and C PCR controls were spotted on a membrane, then incubated with ab231902 at a 1:200 dilution.
Exposure time: 30 seconds.
ab231902 has not yet been referenced specifically in any publications.