Overview

  • Product name

    Anti-5-hydroxymethylcytosine (5-hmC) antibody
    See all 5-hydroxymethylcytosine (5-hmC) primary antibodies
  • Description

    Rabbit polyclonal to 5-hydroxymethylcytosine (5-hmC)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, Dot blotmore details
  • Immunogen

    Chemical/ Small Molecule corresponding to 5-hydroxymethylcytosine (5-hmC) conjugated to keyhole limpet haemocyanin.

Properties

Applications

Our Abpromise guarantee covers the use of ab231902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
Dot blot 1/200.

Target

  • Relevance

    5-Hydroxymethylcytosine (5-hmC) is a modified base form of cytosine recently found in Human/mouse brain and inembryonic stem cells. This DNA pyrimidine nitrogen base can be generated by oxidation of 5-methylcytosine, a reaction mediated by the ten-eleven translocation (TET) family of the 5-mC hydroxylases. The function of this base is still not elucidated but it is believed to play an important role in switching genes on and off.
  • Alternative names

    • 5-hmC antibody

Images

  • An hydroxymethylated DNA IP (hMeDIP) was performed using ab231902.

    IgG isotype antibodies from rabbit were used as negative controls. DNA fragments of 300-500 bp were used. 1 µg of Hela (Human epithelial cell line from cervix adenocarcinoma) cells DNA were spiked with non-methylated, methylated, and hydroxymethylated fragments. The IP’d material has been analysed by qPCR using the primer pair specifc for the 3 different control sequences.

    Lane 1: Unmethylated control.

    Lane 2: Methylated control.

    Lane 3: Hydroxymethylated control.

  • Dot Blot analysis of ab231902 with the C, mC and hmC PCR controls.

    100 to 4 ng (equivalent of 5 to 0.2 pmol of C-bases) of the hmC, mC and C PCR controls were spotted on a membrane, then incubated with ab231902 at a 1:200 dilution.

    Exposure time: 30 seconds.

References

ab231902 has not yet been referenced specifically in any publications.

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