• Product name
    Anti-5-Hydroxymethyluridine antibody
  • Description
    Goat polyclonal to 5-Hydroxymethyluridine
  • Host species
  • Tested applications
    Suitable for: IP, ELISAmore details
  • Immunogen

    5-Hydroxymethyluridine coupled to a carrier protein.



Our Abpromise guarantee covers the use of ab19735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. PubMed: 22514282
ELISA 1/25000.


  • Relevance
    5 Hydroxymethyluridine is formed from thymidine during the oxidation of DNA by ionizing radiation or reactive oxygen species (ROS) that are formed from inflammatory response. This causes genetic mutations, altered gene expression and chromosomal instability that has generally been implicated with aging, cancers and autoimmune disease.
  • Alternative names
    • 5 HmdU antibody
    • 5 Hydroxymethyl 2’ deoxyuridine antibody


This product has been referenced in:
  • Cliffe LJ  et al. JBP1 and 2 are Fe2+/2-OG dependent dioxygenases regulating the hydroxylation of thymidine residues in trypanosome DNA. J Biol Chem : (2012). IP . Read more (PubMed: 22514282) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Thank you for contacting us.

While Dot Blot does indeed have a lot of similarity to a non-reduced/denatured western blot or with an indirect ELISA, we do consider it to be a separate application from either of these. We do however rec...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab19735. To check the status of the order please contact our Customer...

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Thanks for your reply. Actually you have just been corresponding with me regarding this issue. My name is Jeremy and my signature can be found at the bottom of these emails. While you have been sending emails to our general Scientific Support inbox at ...

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Thanks for your reply. I don't believe we discussed the details of your ELISA experiment using ab19735. For our records, please provide a few details regarding the experiment including the sample type and preparation methods and a brief description of ...

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Thank you for your enquiry. We certainly do guarantee our antibodies to work as noted on our online product datasheets. At this time ab19735 is described as having been tested in ELISA. So if the vial of ab19735 is not working in ELISA then I w...

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Thank you for your enquiry. The immunogen used for Goat anti 5-Hydroxymethyluridine, antibody ab19735, was 5-HmUrd coupled to BSA. The 5-HbUrd immunogen was prepared as follows: The 5-HmUrd was dissolved in sodium periodate and reacted for 20 m...

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The product will cross react as follows

5-Hydroxy-2-deoxyuridine (5-HmdUrd) 100%

5-Hydroxymethyluridine (5-HmUrd) 100%

Thymidine (T) 60%

Hydroxymethyluracil (HmU) 43%


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