Overview

  • Product name
    Anti-5 Lipoxygenase / 5-LO (phospho S523) antibody
    See all 5 Lipoxygenase / 5-LO primary antibodies
  • Description
    Rabbit polyclonal to 5 Lipoxygenase / 5-LO (phospho S523)
  • Host species
    Rabbit
  • Specificity
    Specific for the ~80k 5 Lipoxygenase / 5-LO phosphorylated at Ser523 in western blots of rat hippocampal lysates.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human 5 Lipoxygenase/ 5-LO (phospho S523). The exact sequence is proprietary.

  • Positive control
    • WB: Rat cortex tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab30573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 78 kDa).

The immunolabeling is completely eliminated by treatment with lambda-Phosphatase.

Target

  • Function
    Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.
  • Pathway
    Lipid metabolism; leukotriene A4 biosynthesis.
  • Sequence similarities
    Belongs to the lipoxygenase family.
    Contains 1 lipoxygenase domain.
    Contains 1 PLAT domain.
  • Post-translational
    modifications
    Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus.
  • Cellular localization
    Cytoplasm. Nucleus matrix. Nucleus membrane. Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5 Lipoxygenase antibody
    • 5 LO antibody
    • 5 LOX antibody
    • 5-lipoxygenase antibody
    • 5-LO antibody
    • 5-LOX antibody
    • 5LOX antibody
    • 5LPG antibody
    • ALOX 5 antibody
    • Alox5 antibody
    • Arachidonate 5 lipoxygenase antibody
    • Arachidonate 5-lipoxygenase antibody
    • arachidonic 5-lipoxygenase alpha-10 isoform antibody
    • arachidonic 5-lipoxygenase delta-10-13 isoform antibody
    • arachidonic 5-lipoxygenase delta-13 isoform antibody
    • arachidonic 5-lipoxygenase delta-p10 isoform antibody
    • Arachidonic acid 5 lipoxygenase antibody
    • Leukotriene A4 synthase antibody
    • LOG 5 antibody
    • LOG5 antibody
    • LOX5_HUMAN antibody
    • MGC163204 antibody
    see all

Images

  • All lanes : Anti-5 Lipoxygenase / 5-LO (phospho S523) antibody (ab30573) at 1/1000 dilution

    All lanes : rat cortex lysate

    Predicted band size: 78 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?



    Lane 2 was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser523 5Lipoxygenase

References

ab30573 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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