Anti-5 Lipoxygenase/5-LO antibody (ab53514)
Key features and details
- Goat polyclonal to 5 Lipoxygenase/5-LO
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-5 Lipoxygenase/5-LO antibody
See all 5 Lipoxygenase/5-LO primary antibodies -
Description
Goat polyclonal to 5 Lipoxygenase/5-LO -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Hamster -
Immunogen
Synthetic peptide corresponding to Human 5 Lipoxygenase/5-LO aa 651-666 (C terminal).
Sequence:C-ERNKKKQLPYYYLSPD
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Positive control
- Recombinant Human 5 Lipoxygenase/5-LO protein (ab114310) can be used as a positive control in WB. Rat stomach lysate or human bone marrow lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53514 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Notes |
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WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes. -
Pathway
Lipid metabolism; leukotriene A4 biosynthesis. -
Sequence similarities
Belongs to the lipoxygenase family.
Contains 1 lipoxygenase domain.
Contains 1 PLAT domain. -
Post-translational
modificationsSerine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus. -
Cellular localization
Cytoplasm. Nucleus matrix. Nucleus membrane. Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association. - Information by UniProt
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Database links
- Entrez Gene: 100772492 Hamster
- Entrez Gene: 11689 Mouse
- Entrez Gene: 25290 Rat
- SwissProt: P51399 Hamster
- SwissProt: P48999 Mouse
- SwissProt: P12527 Rat
- Unigene: 41072 Mouse
- Unigene: 9662 Rat
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Alternative names
- 5 Lipoxygenase antibody
- 5 LO antibody
- 5 LOX antibody
see all
Images
Datasheets and documents
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Datasheet download
References (0)
ab53514 has not yet been referenced specifically in any publications.