Western blot abreview for Anti-5 Lipoxygenase / 5-LO antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (H2452 Cell line)
Gel Running Conditions
Reduced Denaturing (12% Tris-HEPES-SDS)
Loading amount
20 µg
Specification
H2452 Cell line
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 5% milk block
Dilution
1/400

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: goat anti-rabbit IgG-HRP
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
SuperSignal West Pico Chemiluminescent
Exposure
10 second(s)
Bands
Specific: 70 kDa

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Submitted Sep 28 2009

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