Immunocytochemistry abreview for Anti-5 Lipoxygenase/5-LO antibody

Average
Abreviews
Application
Immunocytochemistry
Sample
Human Cell (Monocytes from Apheresis)
Permeabilization
Yes - 0.1% Triton-X100 in 2% BSA for 15min
Specification
Monocytes from Apheresis
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% BSA in PBS
Dilution
1/100

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Alexa Flour488 Chicken Anti Rabbit IgG (H+L)
Host species: Chicken
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Dr. Mahesh Shivananjappa

Verified customer

Submitted Jan 16 2012

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