5-Maleimidoeosin, Fluorescent triplet probe (ab145355)

Overview

  • Product name

    5-Maleimidoeosin, Fluorescent triplet probe
  • Description

    Fluorescent triplet probe used for the measurement of rotational diffusion of protein in solution and in membranes, including myosin and pyruvate dehydrogenase.
  • Alternative names

    • 5-eosinylmaleimide
    • 5-Maleimido-eosin
    • EMA
    • Eosin-5-maleimide
    • Eosin-5-maleimide
    see all
  • Biological description

    Fluorescent triplet probe used for the measurement of rotational diffusion of protein in solution and in membranes, including myosin and pyruvate dehydrogenase. Widely used in a flow cytometry test to detect hereditary spherocytosis (HS).

  • Purity

    > 93%
  • General notes

    Fluorescence: λex 524 nm; λem 545 nm (after derivatization with 2-mercaptoethanol).

  • CAS Number

    150322-02-4
  • Chemical structure

    Chemical Structure

Properties

  • Excitation

    524nm
  • Emission

    545nm
  • Chemical name

    Eosin-5-maleimide
  • Molecular weight

    742.95
  • Molecular formula

    C24H9Br4NO7
  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in water
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Synthetic

References

This product has been referenced in:

  • Kuzminova JA  et al. [THE CYTOMETRIC TECHNIQUE OF BINDING OF EOSIN-5-MALEIMIDE IN DIAGNOSTIC OF INHERENT SPHEROCYTOSIS]. Klin Lab Diagn 61:168-72 (2016). Read more (PubMed: 27506108) »
  • Joshi P  et al. A comparative evaluation of Eosin-5'-maleimide flow cytometry reveals a high diagnostic efficacy for hereditary spherocytosis. Int J Lab Hematol N/A:N/A (2016). Read more (PubMed: 27339613) »
  • Mehra S  et al. Stability of eosin-5'-maleimide dye used in flow cytometric analysis for red cell membrane disorders. Blood Res 50:109-12 (2015). Read more (PubMed: 26157781) »
  • Tachavanich K  et al. Rapid flow cytometric test using eosin-5-maleimide for diagnosis of red blood cell membrane disorders. Southeast Asian J Trop Med Public Health 40:570-5 (2009). Read more (PubMed: 19842445) »
  • Ishiwata S  et al. Rotational motions of myosin heads in myofibril studied by phosphorescence anisotropy decay measurements. J Biol Chem 262:8314-7 (1987). Read more (PubMed: 2439498) »
  • Visser AJ  et al. Rotational diffusion of eosin-labeled pyruvate dehydrogenase complex of Escherichia coli. Eur J Biochem 121:233-5 (1981). Read more (PubMed: 6173215) »

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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