Product name5-Maleimidoeosin, Fluorescent triplet probe
DescriptionFluorescent triplet probe used for the measurement of rotational diffusion of protein in solution and in membranes, including myosin and pyruvate dehydrogenase.
Fluorescent triplet probe used for the measurement of rotational diffusion of protein in solution and in membranes, including myosin and pyruvate dehydrogenase. Widely used in a flow cytometry test to detect hereditary spherocytosis (HS).
Fluorescence: λex 524 nm; λem 545 nm (after derivatization with 2-mercaptoethanol).
Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in water
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
This product has been referenced in:
- Kuzminova JA et al. [THE CYTOMETRIC TECHNIQUE OF BINDING OF EOSIN-5-MALEIMIDE IN DIAGNOSTIC OF INHERENT SPHEROCYTOSIS]. Klin Lab Diagn 61:168-72 (2016). Read more (PubMed: 27506108) »
- Joshi P et al. A comparative evaluation of Eosin-5'-maleimide flow cytometry reveals a high diagnostic efficacy for hereditary spherocytosis. Int J Lab Hematol N/A:N/A (2016). Read more (PubMed: 27339613) »
- Mehra S et al. Stability of eosin-5'-maleimide dye used in flow cytometric analysis for red cell membrane disorders. Blood Res 50:109-12 (2015). Read more (PubMed: 26157781) »
- Tachavanich K et al. Rapid flow cytometric test using eosin-5-maleimide for diagnosis of red blood cell membrane disorders. Southeast Asian J Trop Med Public Health 40:570-5 (2009). Read more (PubMed: 19842445) »
- Ishiwata S et al. Rotational motions of myosin heads in myofibril studied by phosphorescence anisotropy decay measurements. J Biol Chem 262:8314-7 (1987). Read more (PubMed: 2439498) »
- Visser AJ et al. Rotational diffusion of eosin-labeled pyruvate dehydrogenase complex of Escherichia coli. Eur J Biochem 121:233-5 (1981). Read more (PubMed: 6173215) »