Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805)](https://www.abcam.com/ps/products/10/ab10805/Images/ab10805-18468-anti-5-methylcytosine-5-mc-antibody-33d3-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805)")
Key features and details
- Mouse monoclonal [33D3] to 5-methylcytosine (5-mC)
- Suitable for: IHC-P, IP, Southern Blot, Flow Cyt, IHC-Fr
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-5-methylcytosine (5-mC) antibody [33D3]
See all 5-methylcytosine (5-mC) primary antibodies -
Description
Mouse monoclonal [33D3] to 5-methylcytosine (5-mC) -
Host species
Mouse -
Specificity
Modified base 5-methylcytidine found in DNA of plants and vertebrates.
Raised against the modified ribonucleoside. Specific for the presence of a methyl group on carbon 5 of the pyrimidine ring.
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Tested applications
Suitable for: IHC-P, IP, Southern Blot, Flow Cyt, IHC-Frmore details
Unsuitable for: ICC -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to 5-methylcytosine (5-mC).
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General notes
Storage in frost-free freezers is not recommended. Should this product contain a precipitate microcentrifugation before use.While older lots have performed well in ICC, we have received inconsistent results with the latest lots. Unfortunately, we can no longer guarantee this antibody for use in ICC.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 0.8% Sodium chloride, 0.02% Potassium chloride, 0.03% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
Purity
Protein A purified -
Primary antibody notes
Ab10805 recognises the modified base 5-methylcytidine found in DNA of plants and vertebrates. DNA methylation is a DNA modification process, which is involved in the control of gene expression. Reports suggest that in tumours, DNA is frequently globally hypomethylated compared to the DNA from normal tissue. -
Clonality
Monoclonal -
Clone number
33D3 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10805 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (4) |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| IP | (1) |
Use at an assay dependent concentration.
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| Southern Blot |
1/200.
|
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| Flow Cyt | (1) |
Use at an assay dependent concentration.
Use 10ul of working dilution to label 1000000 cells in 100ul. (see Habib, M. et al. (1999)). |
| IHC-Fr |
Use at an assay dependent concentration.
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| Notes |
|---|
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
IP
Use at an assay dependent concentration. |
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Southern Blot
1/200. |
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Flow Cyt
Use at an assay dependent concentration. Use 10ul of working dilution to label 1000000 cells in 100ul. (see Habib, M. et al. (1999)). |
|
IHC-Fr
Use at an assay dependent concentration. |
Target
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Form
The native context of double-stranded DNA may obstruct antibody binding to 5-methylcytosine. For successful detection of 5-methylcytosine, we recommend that the DNA is denatured to make the nucleotides accessible for the antibody. Denaturing methods vary depending on each application. -
Alternative names
- 5 m C antibody
- 5 mC antibody
- 5 me C antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805)This image is courtesy of an Abreview submitted by Ms Sara Maj Wätjen Hyldig
ab10805 staining 5-Methyl Cytidine in pig embryo (15 to 17 days) tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in paraformaldehyde, heat mediated antigen retrieval in Tris-EDTA buffer, permeabilization in Triton X-100 and blocking in 2% BSA for 10 minutes at 25°C. The primary antibody was diluted, 1/100 (PBS + 2% BSA) and incubated with sample for 1 hour at 25°C. An Alexa Fluor® 488 conjugated donkey polyclonal to mouse at 1/250 dilution, was used as secondary.
Protocols
References (103)
ab10805 has been referenced in 103 publications.
- Bettencourt C et al. White matter DNA methylation profiling reveals deregulation of HIP1, LMAN2, MOBP, and other loci in multiple system atrophy. Acta Neuropathol 139:135-156 (2020). PubMed: 31535203
- Chen L et al. PHLPP2 is a novel biomarker and epigenetic target for the treatment of vitamin C in pancreatic cancer. Int J Oncol 56:1294-1303 (2020). PubMed: 32319585
- Mei L et al. RNA methyltransferase NSUN2 promotes gastric cancer cell proliferation by repressing p57Kip2 by an m5C-dependent manner. Cell Death Dis 11:270 (2020). PubMed: 32332707
- Salmani S et al. Testosterone amplifies HSP70-2a, HSP90 and PCNA expression in experimental varicocele condition: Implication for DNA fragmentation. Reprod Biol N/A:N/A (2020). PubMed: 32461192
- Jiang Q et al. DNA Methylation Inhibits the Expression of CFSH in Mud Crab. Front Endocrinol (Lausanne) 11:163 (2020). PubMed: 32328029
