Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805)](https://www.abcam.com/ps/products/10/ab10805/Images/ab10805-18468-anti-5-methylcytosine-5-mc-antibody-33d3-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805)")
Key features and details
- Mouse monoclonal [33D3] to 5-methylcytosine (5-mC)
- Suitable for: IHC-P, IP, Southern Blot, Flow Cyt, IHC-Fr
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-5-methylcytosine (5-mC) antibody [33D3]
See all 5-methylcytosine (5-mC) primary antibodies -
Description
Mouse monoclonal [33D3] to 5-methylcytosine (5-mC) -
Host species
Mouse -
Specificity
Modified base 5-methylcytidine found in DNA of plants and vertebrates.
Raised against the modified ribonucleoside. Specific for the presence of a methyl group on carbon 5 of the pyrimidine ring.
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Tested applications
Suitable for: IHC-P, IP, Southern Blot, Flow Cyt, IHC-Frmore details
Unsuitable for: ICC -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to 5-methylcytosine (5-mC).
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General notes
Storage in frost-free freezers is not recommended. Should this product contain a precipitate microcentrifugation before use.While older lots have performed well in ICC, we have received inconsistent results with the latest lots. Unfortunately, we can no longer guarantee this antibody for use in ICC.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 0.4% PBS, 0.8% Sodium chloride -
Concentration information loading... -
Purity
Protein A purified -
Primary antibody notes
Ab10805 recognises the modified base 5-methylcytidine found in DNA of plants and vertebrates. DNA methylation is a DNA modification process, which is involved in the control of gene expression. Reports suggest that in tumours, DNA is frequently globally hypomethylated compared to the DNA from normal tissue. -
Clonality
Monoclonal -
Clone number
33D3 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10805 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (4) |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| IP | (1) |
Use at an assay dependent concentration.
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| Southern Blot |
1/200.
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| Flow Cyt | (1) |
Use at an assay dependent concentration.
Use 10ul of working dilution to label 1000000 cells in 100ul. (see Habib, M. et al. (1999)). |
| IHC-Fr |
Use at an assay dependent concentration.
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| Notes |
|---|
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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IP
Use at an assay dependent concentration. |
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Southern Blot
1/200. |
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Flow Cyt
Use at an assay dependent concentration. Use 10ul of working dilution to label 1000000 cells in 100ul. (see Habib, M. et al. (1999)). |
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IHC-Fr
Use at an assay dependent concentration. |
Target
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Form
The native context of double-stranded DNA may obstruct antibody binding to 5-methylcytosine. For successful detection of 5-methylcytosine, we recommend that the DNA is denatured to make the nucleotides accessible for the antibody. Denaturing methods vary depending on each application. -
Alternative names
- 5 m C antibody
- 5 mC antibody
- 5 me C antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805)This image is courtesy of an Abreview submitted by Ms Sara Maj Wätjen Hyldig
ab10805 staining 5-Methyl Cytidine in pig embryo (15 to 17 days) tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in paraformaldehyde, heat mediated antigen retrieval in Tris-EDTA buffer, permeabilization in Triton X-100 and blocking in 2% BSA for 10 minutes at 25°C. The primary antibody was diluted, 1/100 (PBS + 2% BSA) and incubated with sample for 1 hour at 25°C. An Alexa Fluor® 488 conjugated donkey polyclonal to mouse at 1/250 dilution, was used as secondary.
Protocols
Datasheets and documents
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Datasheet download
References (127)
ab10805 has been referenced in 127 publications.
- Zhu X et al. Ubiquitination-mediated degradation of TRDMT1 regulates homologous recombination and therapeutic response. NAR Cancer 3:zcab010 (2021). PubMed: 33778494
- Chen X et al. DNA Hypermethylation Induced by L-Methionine Leads to Oligodendroglial and Myelin Deficits and Schizophrenia-Like Behaviors in Adolescent Mice. Front Neurosci 15:659853 (2021). PubMed: 33958986
- Wolny E et al. Defining the Cell Wall, Cell Cycle and Chromatin Landmarks in the Responses of Brachypodium distachyon to Salinity. Int J Mol Sci 22:N/A (2021). PubMed: 33477958
- Li M et al. Regulation of MYB by distal enhancer elements in human myeloid leukemia. Cell Death Dis 12:223 (2021). PubMed: 33637692
- Biondi LR et al. Quantification of Global DNA Methylation in Canine Mammary Gland Tumors via Immunostaining of 5-Methylcytosine: Histopathological and Clinical Correlations. Front Vet Sci 8:628241 (2021). PubMed: 33718471
