For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    5-methylcytosine-5-mc-antibody-33d3-ab10805.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling DNA methylation Methylated DNA
Share by email

Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805)

  • Datasheet
Reviews (12)Q&A (18)References (127)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805)

    Key features and details

    • Mouse monoclonal [33D3] to 5-methylcytosine (5-mC)
    • Suitable for: IHC-P, IP, Southern Blot, Flow Cyt, IHC-Fr
    • Reacts with: Species independent
    • Isotype: IgG1

    You may also be interested in

    Primary
    Anti-5-Hydroxymethyluridine antibody (ab19735)
    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    Primary
    Product image
    Anti-5-hydroxymethylcytosine (5-hmC) antibody [RM236] (ab214728)

    View more associated products

    Overview

    • Product name

      Anti-5-methylcytosine (5-mC) antibody [33D3]
      See all 5-methylcytosine (5-mC) primary antibodies
    • Description

      Mouse monoclonal [33D3] to 5-methylcytosine (5-mC)
    • Host species

      Mouse
    • Specificity

      Modified base 5-methylcytidine found in DNA of plants and vertebrates.

      Raised against the modified ribonucleoside. Specific for the presence of a methyl group on carbon 5 of the pyrimidine ring.

    • Tested applications

      Suitable for: IHC-P, IP, Southern Blot, Flow Cyt, IHC-Frmore details
      Unsuitable for: ICC
    • Species reactivity

      Reacts with: Species independent
    • Immunogen

      Chemical/ Small Molecule corresponding to 5-methylcytosine (5-mC).

    • General notes

      Storage in frost-free freezers is not recommended. Should this product contain a precipitate microcentrifugation before use.While older lots have performed well in ICC, we have received inconsistent results with the latest lots. Unfortunately, we can no longer guarantee this antibody for use in ICC.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Constituents: 0.4% PBS, 0.8% Sodium chloride
    • Concentration information loading...
    • Purity

      Protein A purified
    • Primary antibody notes

      Ab10805 recognises the modified base 5-methylcytidine found in DNA of plants and vertebrates. DNA methylation is a DNA modification process, which is involved in the control of gene expression. Reports suggest that in tumours, DNA is frequently globally hypomethylated compared to the DNA from normal tissue.
    • Clonality

      Monoclonal
    • Clone number

      33D3
    • Myeloma

      Sp2/0-Ag14
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Epigenetics and Nuclear Signaling
      • DNA methylation
      • Methylated DNA
      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA / Nucleotides

    Associated products

    • ChIP Related Products

      • ChIP Kit (ab500)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab10805 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P (4)
    Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    IP (1)
    Use at an assay dependent concentration.
    Southern Blot
    1/200.
    Flow Cyt (1)
    Use at an assay dependent concentration.

    Use 10ul of working dilution to label 1000000 cells in 100ul. (see Habib, M. et al. (1999)).
    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    IHC-Fr
    Use at an assay dependent concentration.
    Notes
    IHC-P
    Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    IP
    Use at an assay dependent concentration.
    Southern Blot
    1/200.
    Flow Cyt
    Use at an assay dependent concentration.

    Use 10ul of working dilution to label 1000000 cells in 100ul. (see Habib, M. et al. (1999)).
    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    IHC-Fr
    Use at an assay dependent concentration.
    Application notes
    Is unsuitable for ICC.

    Target

    • Form

      The native context of double-stranded DNA may obstruct antibody binding to 5-methylcytosine. For successful detection of 5-methylcytosine, we recommend that the DNA is denatured to make the nucleotides accessible for the antibody. Denaturing methods vary depending on each application.
    • Alternative names

      • 5 m C antibody
      • 5 mC antibody
      • 5 me C antibody
      • 5 Me Cytidine antibody
      • 5 Me Cytosine antibody
      • 5 meC antibody
      • 5 Methycytosine antibody
      • 5 Methyl Cytidine antibody
      • 5 Methyl Cytosine antibody
      • 5 Methyl-Cytidine antibody
      • 5 Methyl-Cytosine antibody
      • 5 MethylCytidine antibody
      • 5 MethylCytosine antibody
      • 5-mC antibody
      • 5-Me Cytidine antibody
      • 5-Me Cytosine antibody
      • 5-meC antibody
      • 5-Methyl Cytidine antibody
      • 5-Methyl-Cytidine antibody
      • 5-Methyl-Cytosine antibody
      • 5-MethylCytidine antibody
      • 5-MethylCytosine antibody
      • 5mC antibody
      • methyl CpG antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805)This image is courtesy of an Abreview submitted by Ms Sara Maj Wätjen Hyldig

      ab10805 staining 5-Methyl Cytidine in pig embryo (15 to 17 days) tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in paraformaldehyde, heat mediated antigen retrieval in Tris-EDTA buffer, permeabilization in Triton X-100 and blocking in 2% BSA for 10 minutes at 25°C. The primary antibody was diluted, 1/100 (PBS + 2% BSA) and incubated with sample for 1 hour at 25°C. An Alexa Fluor® 488 conjugated donkey polyclonal to mouse at 1/250 dilution, was used as secondary.

      See Abreview

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (127)

    Publishing research using ab10805? Please let us know so that we can cite the reference in this datasheet.

    ab10805 has been referenced in 127 publications.

    • Zhu X  et al. Ubiquitination-mediated degradation of TRDMT1 regulates homologous recombination and therapeutic response. NAR Cancer 3:zcab010 (2021). PubMed: 33778494
    • Chen X  et al. DNA Hypermethylation Induced by L-Methionine Leads to Oligodendroglial and Myelin Deficits and Schizophrenia-Like Behaviors in Adolescent Mice. Front Neurosci 15:659853 (2021). PubMed: 33958986
    • Wolny E  et al. Defining the Cell Wall, Cell Cycle and Chromatin Landmarks in the Responses of Brachypodium distachyon to Salinity. Int J Mol Sci 22:N/A (2021). PubMed: 33477958
    • Li M  et al. Regulation of MYB by distal enhancer elements in human myeloid leukemia. Cell Death Dis 12:223 (2021). PubMed: 33637692
    • Biondi LR  et al. Quantification of Global DNA Methylation in Canine Mammary Gland Tumors via Immunostaining of 5-Methylcytosine: Histopathological and Clinical Correlations. Front Vet Sci 8:628241 (2021). PubMed: 33718471
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 30 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-5-methylcytosine (5-mC) antibody [33D3]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Lung bronchial biopsy)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    Lung bronchial biopsy
    Blocking step
    20% FCS, 1% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
    Fixative
    GMA-embedded
    Read More

    Abcam user community

    Verified customer

    Submitted May 23 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-5-methylcytosine (5-mC) antibody [33D3]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (18dpc Male Urogenital Sinus)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate, pH 6
    Permeabilization
    No
    Specification
    18dpc Male Urogenital Sinus
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 28 2017

    Flow Cytometry abreview for Anti-5-methylcytosine (5-mC) antibody [33D3]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Zebrafish Cell (Dissociated tissue cells)
    Permeabilization
    Yes - Methanol 90%
    Gating Strategy
    Cells were gated according to their DNA content
    Specification
    Dissociated tissue cells
    Preparation
    Cell harvesting/tissue preparation method: Tissue were dissociated with collagenase. Obtained cells were filtered and fixed.
    Sample buffer: Cells were analysed in PBS
    Fixation
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 01 2017

    Other abreview for Anti-5-methylcytosine (5-mC) antibody [33D3]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Other - Do not use
    Sample
    Zebrafish Tissue lysate - other (5 dpf embryo)
    Read More

    Kathryn Bambino

    Verified customer

    Submitted Feb 03 2015

    Immunoprecipitation abreview for Anti-5-Methyl Cytidine antibody [33D3] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    Application
    Immunoprecipitation
    Sample
    Rat Tissue lysate - other (mammary gland)
    Total protein in input
    20 µg
    Specification
    mammary gland
    Treatment
    maternal high fat
    Immuno-precipitation step
    Other - Dynabeads
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 13 2013

    ChIP abreview for Anti-5-methylcytosine (5-mC) antibody [33D3]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Mouse Cell lysate - nuclear (Mouse ES cells)
    Negative control
    Magoh2 in WT cells
    Specification
    Mouse ES cells
    Detection step
    Real-time PCR
    Type
    Native ChIP (N-ChIP)
    Positive control
    Magoh2 in HMT KO cells
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 03 2012

    Question

    Inquiry: Does the antibody recognise m5C in RNA? Is it then still specific to the modification?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 23 2016

    Answer

    ab10805 specifically binds to single modified cytosine so it should bind to 5-Methylcytosine in RNA as well.

    Read More

    Padamjeet Singh

    Abcam Scientific Support

    Answered on Jun 23 2016

    Question

    Can the Anti-5-Methyl Cytidine antibody [33D3] - ChIP Grade (ab10805) distinguish 5-mc and 5-hmc?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 03 2012

    Answer

    Thank you for contacting us. While we have no tested the specificity for 5-hmC in house, customers have reported that this clone does not recognize 5-hmC. Unfortunately, we do not currently have any data regarding this reactivity.

    Read More

    Abcam Scientific Support

    Answered on Dec 03 2012

    Question

    Inquiry: Hello We have found a product in your catalog: Anti-5-Methyl Cytidine antibody [33D3] - ChIP Grade (ab10805) You also have a wide choice of secondary antibody IgG (FITC). Can you please advice which of them best suits to the ab 10805? Thank you in advance,

    Read More

    Abcam community

    Verified customer

    Asked on Nov 12 2012

    Answer

    Thank you for contacting us.

    There are many anti mouse secondary FITC conjugated antibodies available - they are all fine. Please buy the following antibody; https://www.abcam.com/goat-polyclonal-secondary-antibody-to-mouse-igg1-heavy-chain-fitc-pre-adsorbed-ab98692.html

    This antibody is a preadsorbed one. Please click the link for more information; https://www.abcam.com/index.html?pageconfig=resource&rid=12956

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Nov 12 2012

    Question

    Hi, I am currently using your ab10805 (anti-5mC) antibody. Does this antibody recognize a single 5mC or recognize an oligonucleotide sequence with 5mC in between?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 21 2012

    Answer

    Thank you for contacting us.

    ab10805 should recognize single 5-methyl cytidine.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Aug 21 2012

    1-10 of 30 Abreviews or Q&A

    •  Previous
    • 1
    • 2
    • 3
    • Next 

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2022 Abcam plc. All rights reserved.