Anti-5-methylcytosine (5-mC) antibody [5MC-CD] (ab73938)
Key features and details
- Mouse monoclonal [5MC-CD] to 5-methylcytosine (5-mC)
- Suitable for: Southern Blot, ICC/IF
- Reacts with: Species independent
- Isotype: IgM
Related conjugates and formulations
Overview
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Product name
Anti-5-methylcytosine (5-mC) antibody [5MC-CD]
See all 5-methylcytosine (5-mC) primary antibodies -
Description
Mouse monoclonal [5MC-CD] to 5-methylcytosine (5-mC) -
Host species
Mouse -
Specificity
ab73938 reacts with DNA with 5-Methylcytosine (methylated DNA). -
Tested applications
Suitable for: Southern Blot, ICC/IFmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to 5-methylcytosine (5-mC) conjugated to Bovine Serum Albumin (BSA).
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Positive control
- Chlamidomonas me-1 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purification notes
ab73938 is purified and filter-sterilised. -
Clonality
Monoclonal -
Clone number
5MC-CD -
Isotype
IgM -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab73938 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Southern Blot |
1/1000.
Immunoblotting - detection of DNA with 5-methylocytosine on nitrocellulose. Use at 1/1000 |
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ICC/IF | (1) |
1/50 - 1/100.
Recommend fixing in 4% PFA followed by denaturation with 70% formamide in 2x SSC and chilling in 70%, 95%, and 99% EtOH for 2 min each. Diluting antibody in SSC (saline sodium citrate) may give optimal results. See protocols from PubMed: 11983892 and 17994007 |
Notes |
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Southern Blot
1/1000. Immunoblotting - detection of DNA with 5-methylocytosine on nitrocellulose. Use at 1/1000 |
ICC/IF
1/50 - 1/100. Recommend fixing in 4% PFA followed by denaturation with 70% formamide in 2x SSC and chilling in 70%, 95%, and 99% EtOH for 2 min each. Diluting antibody in SSC (saline sodium citrate) may give optimal results. See protocols from PubMed: 11983892 and 17994007 |
Target
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Form
The native context of double-stranded DNA may obstruct antibody binding to 5-methylcytosine. For successful detection of 5-methylcytosine, we recommend that the DNA is denatured to make the nucleotides accessible for the antibody. Denaturing methods vary depending on each application. -
Alternative names
- 5 m C antibody
- 5 mC antibody
- 5 me C antibody
see all
Images
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Methylation of chloroplast DNA visualized by immunochemistry. Samples are Chlamidomonas me-1 cells. Left: DAPI-stained cells. Middle: Cells stained with ab73938 and FITC-conjugated 2nd antibody. Right: Merged image. Chloroplast DNA is exclusively methylated in gamete cells
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ab73938 staining 5-Methyl Cytosine in murine PN5 zygotes by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with methanol/acetic acid (3:1) and blocked with 30% serum for overnight a 4°C. Samples were incubated with primary antibody (1/100 in diluent) for 18 hours at 4°C before detection with a fluorescent secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (29)
ab73938 has been referenced in 29 publications.
- Bara AW et al. DNA Methylation-An Epigenetic Mark in Mutagen-Treated Brachypodium distachyon Cells. Plants (Basel) 10:N/A (2021). PubMed: 34371611
- Park H et al. Rad50 mediates DNA demethylation to establish pluripotent reprogramming. Exp Mol Med 52:1116-1127 (2020). PubMed: 32665583
- Zhang Z et al. A twin-pair analysis indicates congenital scoliosis is associated with allele-specific methylation in the SVIL gene. Mol Med Rep 22:2093-2100 (2020). PubMed: 32582973
- Kornicka K et al. 5-Azacytydine and resveratrol reverse senescence and ageing of adipose stem cells via modulation of mitochondrial dynamics and autophagy. J Cell Mol Med 23:237-259 (2019). PubMed: 30370650
- Marycz K et al. 5-Azacytidine and Resveratrol Enhance Chondrogenic Differentiation of Metabolic Syndrome-Derived Mesenchymal Stem Cells by Modulating Autophagy. Oxid Med Cell Longev 2019:1523140 (2019). PubMed: 31214275