For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
DNA with 5-Methylcytosine antibody [5MC-CD] (ab73938) How did you confirm the specificity of this antibody? Thank you for your time and help in advance.
Asked on Aug 04 2010
Thank you for your enquiry. The specificity of the anti-5Methylcytosine antibody has been tested in many published papers included articles by Prof. H. Sano who has done a lot of work on this antibody. Some of them were cited in the references in the datasheet. I recommend to review these for further information. Further references would be available by PubMed search with key words 'Sano H, 5Methyytosine', or 'DNA methylation'. From recent studies, methylated cytosine protrude from the duplex, therefore the antibody may be able to recognize metylation on both native duplex and denatured DNA. I am sorry we have no additional data at the moment. From the online datasheet: Sano H et al. Detection of heavy methylation in human repetitive DNA subsets by a monoclonal antibody against 5-methylcytosine. Biochim Biophys Acta 951:157-65 (1988). PubMed: 2847796 Additionally: Sano H et al “Identification of 5-methycytosine in DNA fragment immobilized on nitrocellulose paper “PNAS 77:3581-3585 (1980) PMID: 6251470 I hope this will be helpful to you. Should you have any further questions, please do not hesitate to contact us.
Answered on Aug 04 2010