Immunocytochemistry/ Immunofluorescence abreview for Anti-5-Methyl Cytosine antibody [5MC-CD]

Poor
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton-X100 in PBS

Other product details

Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-Mouse Alexafluor488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200

Additional data

Additional Notes
Asynchronous cells were paraformaldehyde-fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. This antibody produces a weak staining pattern that is only slightly enriched within nucle - it appears to be predominantly non-specific staining. MeOH-fixed samples were also evaluated and failed to produce the expected staining pattern.
Abcam response
This antibody has been successfully used for ICC/IF under different experimental conditions. The fact that you have not been able to get a good result with this antibody might imply that not all protocols will be equally suitable and that the results obtained are highly dependent on the method used. Thank you for your time. We hope that this information will be helpful for other customers.

Dr. Kirk Mcmanus

Verified customer

Submitted Feb 07 2012

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