Key features and details
- Mouse monoclonal [5MC-CD] to 5-methylcytosine (5-mC) - Azide free (Biotin)
- Suitable for: Southern Blot, ICC/IF
- Reacts with: Mouse, a wide range of other species
- Conjugation: Biotin
- Isotype: IgM
Product nameAnti-5-methylcytosine (5-mC) antibody [5MC-CD] - Azide free (Biotin)
See all 5-methylcytosine (5-mC) primary antibodies
DescriptionMouse monoclonal [5MC-CD] to 5-methylcytosine (5-mC) - Azide free (Biotin)
Tested applicationsSuitable for: Southern Blot, ICC/IFmore details
Species reactivityReacts with: Mouse, a wide range of other species
Chemical/ Small Molecule corresponding to 5-methylcytosine (5-mC) conjugated to Bovine Serum Albumin (BSA).
- Chlamydomonas me-1 and mouse embryonic stem cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6
Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab179914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Immuno-blotting detection of DNA with 5-methylcytosine on nitrocellulose.
|ICC/IF||1/50 - 1/100.|
FormThe native context of double-stranded DNA may obstruct antibody binding to 5-methylcytosine. For successful detection of 5-methylcytosine, we recommend that the DNA is denatured to make the nucleotides accessible for the antibody. Denaturing methods vary depending on each application.
- 5 m C antibody
- 5 mC antibody
- 5 me C antibody
Immunofluorescence analysis of Clamydomonas me-1 cells, labeling 5-Methyl Cytosine using ab179914 at a 1/50 dilution, followed by FITC-conjugated secondary antibody (green). DAPI was used to stain DNA (magenta). Upper panels (a, b and c) correspond to vegetative cells and lower panels (d, e and f) to gametes.
Immunofluorescence analysis of mouse embryonic cells, labeling 5-Methyl Cytosine using ab179914 at a 1/50 dilution. DAPI was used to stain DNA (magenta).
ab179914 has not yet been referenced specifically in any publications.