Key features and details
- Mouse monoclonal [5MC-CD] to 5-methylcytosine (5-mC) (FITC)
- Suitable for: Southern Blot, ICC/IF
- Reacts with: a wide range of other species
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgM
Product nameAnti-5-methylcytosine (5-mC) antibody [5MC-CD] (FITC)
See all 5-methylcytosine (5-mC) primary antibodies
DescriptionMouse monoclonal [5MC-CD] to 5-methylcytosine (5-mC) (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
Tested applicationsSuitable for: Southern Blot, ICC/IFmore details
Species reactivityReacts with: a wide range of other species
Chemical/ Small Molecule corresponding to 5-methylcytosine (5-mC) conjugated to Bovine Serum Albumin (BSA).
- Mouse embryonic stem cells; Chlamidomonas me-1 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 6
Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS
Concentration information loading...
Purification notesab179898 is filter-sterilised.
Our Abpromise guarantee covers the use of ab179898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Immuno-blotting detection of DNA with 5-methylcytosine on nitrocellulose.
|ICC/IF||1/50 - 1/100.|
FormThe native context of double-stranded DNA may obstruct antibody binding to 5-methylcytosine. For successful detection of 5-methylcytosine, we recommend that the DNA is denatured to make the nucleotides accessible for the antibody. Denaturing methods vary depending on each application.
- 5 m C antibody
- 5 mC antibody
- 5 me C antibody
Immunofluorescent analysis of Chlamidomonas me-1 cells, labeling 5-Methyl Cytosine with ab179898 at 1/50 dilution, followed by a FITC-conjugated secondary antibody (middle panel). Left: DAPI-stained cells. Right: Merged image. Chloroplast DNA is exclusively methylated in gamete cells (bottom row), as opposed to vegetative cells (top row).
Immunofluorescent analysis of mouse embryonic stem cells labeling 5-Methyl Cytosine with ab179898 at 1/50 dilution.
ab179898 has not yet been referenced specifically in any publications.