5-TAMRA (5-Carboxytetramethylrhodamine), Fluorescent dye for DNA, peptides and proteins (ab145438)

Overview

  • Product name

    5-TAMRA (5-Carboxytetramethylrhodamine), Fluorescent dye for DNA, peptides and proteins
  • Description

    Fluorescent dye for DNA, peptides and proteins. Amine-reactive.
  • Biological description

    Fluorescent dye for DNA, peptides and proteins. Amine-reactive. Used for oligonucleotide labeling and automated DNA sequencing applications. TAMRA is often used as FRET acceptor for FAM fluorophore. Free 5(6)-TAMRA (ab145282) and 6-TAMRA (ab145338) isomer. 5-TAMRA N-succinimidyl ester (ab145433) precursor.

  • Purity

    > 97%
  • CAS Number

    91809-66-4
  • Chemical structure

    Chemical Structure

Properties

  • Excitation

    546nm
  • Emission

    579nm
  • Chemical name

    5-Carboxytetramethylrhodamine
  • Molecular weight

    430.45
  • Molecular formula

    C25H22N2O5
  • Storage instructions

    Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in DMSO
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    CN(C)c4cc5Oc1cc(ccc1C3(OC(=O)c2cc(ccc23)C(=O)O)c5cc4)N(C)C
  • Source

    Synthetic

  • Research areas

References

This product has been referenced in:

  • Eisold U  et al. Bright or dark immune complexes of anti-TAMRA antibodies for adapted fluorescence-based bioanalysis. Anal Bioanal Chem 407:3313-23 (2015). Read more (PubMed: 25711988) »
  • Zhu P  et al. Fluorescence quenching by TEMPO: a sub-30 A single-molecule ruler. Biophys J 89:L37-9 (2005). Read more (PubMed: 16199509) »
  • Vámosi G  et al. Fluorescence characteristics of 5-carboxytetramethylrhodamine linked covalently to the 5' end of oligonucleotides: multiple conformers of single-stranded and double-stranded dye-DNA complexes. Biophys J 71:972-94 (1996). Read more (PubMed: 8842236) »

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