Product nameAnti-50S ribosomal protein L7/L12 antibody
DescriptionRabbit polyclonal to 50S ribosomal protein L7/L12
Tested applicationsSuitable for: WBmore details
Predicted to work with: Staphylococcus aureus
Recombinant full length protein (His-tag) corresponding to Staphylococcus aureus 50S ribosomal protein L7/L12 aa 1-122. (6xHis-SUMO tag at the N-terminal).
MANHEQIIEAIKEMSVLELNDLVKAIEEEFGVTAAAPVAVAGAAGGADAA AEKTEFDVELTSAGSSKIKVVKAVKEATGLGLKDAKELVDGAPKVIKEAL PKEEAEKLKEQLEEVGATVELK
Database link: P66061
- WB: Recombinant Staphylococcus aureus 50S ribosomal protein L7/L12 protein with 6xHis-SUMO tag at the N-terminal.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.03% Proclin
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab225681 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Detects a band of approximately 29 kDa (predicted molecular weight: 13 kDa).|
RelevanceFunction: Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Is thus essential for accurate translation.
- rplL antibody
All lanes : Anti-50S ribosomal protein L7/L12 antibody (ab225681) at 1/500 dilution
Lane 1 : Recombinant Staphylococcus aureus 50S ribosomal protein L7/L12 protein with 6xHis-SUMO tag at the N-terminal at 0.08 µg
Lane 2 : Recombinant Staphylococcus aureus 50S ribosomal protein L7/L12 protein with 6xHis-SUMO tag at the N-terminal at 0.04 µg
Lane 3 : Recombinant Staphylococcus aureus 50S ribosomal protein L7/L12 protein with 6xHis-SUMO tag at the N-terminal at 0.025 µg
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 13 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?
Predicted MWt including 6xHis-SUMO tag: 29 kDa
ab225681 has not yet been referenced specifically in any publications.