Key features and details
- Rabbit polyclonal to 50S ribosomal protein L7/L12
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
Product nameAnti-50S ribosomal protein L7/L12 antibody
DescriptionRabbit polyclonal to 50S ribosomal protein L7/L12
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Recombinant fragment
Predicted to work with: Staphylococcus aureus
Recombinant full length protein (His-tag) corresponding to Staphylococcus aureus 50S ribosomal protein L7/L12 aa 1-122. (6xHis-SUMO tag at the N-terminal).
MANHEQIIEAIKEMSVLELNDLVKAIEEEFGVTAAAPVAVAGAAGGADAA AEKTEFDVELTSAGSSKIKVVKAVKEATGLGLKDAKELVDGAPKVIKEAL PKEEAEKLKEQLEEVGATVELK
Database link: P66061
- WB: Recombinant Staphylococcus aureus 50S ribosomal protein L7/L12 protein with 6xHis-SUMO tag at the N-terminal.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab225681 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Detects a band of approximately 29 kDa (predicted molecular weight: 13 kDa).|
RelevanceFunction: Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Is thus essential for accurate translation.
- rplL antibody
All lanes : Anti-50S ribosomal protein L7/L12 antibody (ab225681) at 1/500 dilution
Lane 1 : Recombinant Staphylococcus aureus 50S ribosomal protein L7/L12 protein with 6xHis-SUMO tag at the N-terminal at 0.08 µg
Lane 2 : Recombinant Staphylococcus aureus 50S ribosomal protein L7/L12 protein with 6xHis-SUMO tag at the N-terminal at 0.04 µg
Lane 3 : Recombinant Staphylococcus aureus 50S ribosomal protein L7/L12 protein with 6xHis-SUMO tag at the N-terminal at 0.025 µg
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 13 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?
Predicted MWt including 6xHis-SUMO tag: 29 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225681 has not yet been referenced specifically in any publications.