Overview

  • Product name
  • Description
    Rabbit polyclonal to 53BP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IP, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human 53BP1 aa 1400-1700.

Properties

Applications

Our Abpromise guarantee covers the use of ab172580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 214 kDa.
ICC/IF 1/1000.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/1000.

Target

  • Function
    May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.
  • Involvement in disease
    Note=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein.
  • Sequence similarities
    Contains 2 BRCT domains.
  • Post-translational
    modifications
    Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
    Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation.
  • Cellular localization
    Nucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks.
  • Information by UniProt
  • Database links
  • Alternative names
    • 53 BP1 antibody
    • 53BP1 antibody
    • FLJ41424 antibody
    • MGC138366 antibody
    • p202 antibody
    • p53 binding protein 1 antibody
    • p53 BP1 antibody
    • p53-binding protein 1 antibody
    • p53BP1 antibody
    • TP53 BP1 antibody
    • TP53B_HUMAN antibody
    • Tp53bp1 antibody
    • TRP53 BP1 antibody
    • Tumor protein 53 binding protein 1 antibody
    • Tumor protein p53 binding protein 1 antibody
    • Tumor suppressor p53 binding protein 1 antibody
    • Tumor suppressor p53-binding protein 1 antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling 53BP1 with ab172580 at 1:1000. Cells were fixed with PFA and permeabilized with methanol for 2 minutes. Cells were incubated with the primary anitbody overnight at room temperature. Top - 53BP1, Middle - DAPI, Bottom - Merge.

  • Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling 53BP1 with ab172580 at 1:1000. Cells were fixed with PFA and permeabilized with methanol for 2 minutes. Cells were incubated with the primary anitbody overnight at room temperature.

  • Flow Cytometry analysis of HeLa cells labelling 53BP1 with ab172580 at 1:800. Cells were fixed with 1% PFA. Cells were incuabted with the primary antibody overnight at 4°C.

  • Immunohistochemistry (formalin/PFA-fixed paraffin-embedded) analysis of Human linfoid tissue labelling 53BP1 with ab172580 at 1:1000. Antigen retrieval was by microwave boiling for 7 minutes in 10mM TrisHCl, pH9.

  • Immunoprecipitation analysis of U2OS cells labelling 53BP1 with ab172580. Protein A beads were crosslinked to the serum.

  • All lanes : Anti-53BP1 antibody (ab172580)

    Lane 1 : Control si
    Lane 2 : 53BP1 si
    Lane 3 : NIH 3T3 cell lysate at 40 µg
    Lane 4 : PC12 cell lysate at 40 µg

    Predicted band size: 214 kDa

References

This product has been referenced in:
  • Moruno-Manchon JF  et al. Inhibiting sphingosine kinase 2 mitigates mutant Huntingtin-induced neurodegeneration in neuron models of Huntington disease. Hum Mol Genet 26:1305-1317 (2017). Read more (PubMed: 28175299) »
  • Kovatcheva M  et al. ATRX is a regulator of therapy induced senescence in human cells. Nat Commun 8:386 (2017). ICC/IF ; Human . Read more (PubMed: 28855512) »
See all 7 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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