Anti-53BP1 antibody (ab172580)
Key features and details
- Rabbit polyclonal to 53BP1
- Suitable for: WB, ICC/IF, IP, Flow Cyt, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-53BP1 antibody
See all 53BP1 primary antibodies -
Description
Rabbit polyclonal to 53BP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IP, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human 53BP1 aa 1400-1700.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. -
Storage buffer
Constituent: Water -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab172580 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 214 kDa. | |
| ICC/IF | 1/1000. | |
| IP | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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| IHC-P | 1/1000. |
Target
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Function
May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage. -
Involvement in disease
Note=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein. -
Sequence similarities
Contains 2 BRCT domains. -
Post-translational
modificationsAsymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation. -
Cellular localization
Nucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks. - Information by UniProt
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Database links
- Entrez Gene: 7158 Human
- Entrez Gene: 27223 Mouse
- Entrez Gene: 296099 Rat
- Omim: 605230 Human
- SwissProt: Q12888 Human
- SwissProt: P70399 Mouse
- Unigene: 440968 Human
- Unigene: 383499 Mouse
see all -
Alternative names
- 53 BP1 antibody
- 53BP1 antibody
- FLJ41424 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-53BP1 antibody (ab172580)
Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling 53BP1 with ab172580 at 1:1000. Cells were fixed with PFA and permeabilized with methanol for 2 minutes. Cells were incubated with the primary anitbody overnight at room temperature. Top - 53BP1, Middle - DAPI, Bottom - Merge.
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Immunocytochemistry/ Immunofluorescence - Anti-53BP1 antibody (ab172580)Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling 53BP1 with ab172580 at 1:1000. Cells were fixed with PFA and permeabilized with methanol for 2 minutes. Cells were incubated with the primary anitbody overnight at room temperature.
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Flow Cytometry - Anti-53BP1 antibody (ab172580)Flow Cytometry analysis of HeLa cells labelling 53BP1 with ab172580 at 1:800. Cells were fixed with 1% PFA. Cells were incuabted with the primary antibody overnight at 4°C.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 antibody (ab172580)Immunohistochemistry (formalin/PFA-fixed paraffin-embedded) analysis of Human linfoid tissue labelling 53BP1 with ab172580 at 1:1000. Antigen retrieval was by microwave boiling for 7 minutes in 10mM TrisHCl, pH9.
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Immunoprecipitation - Anti-53BP1 antibody (ab172580)Immunoprecipitation analysis of U2OS cells labelling 53BP1 with ab172580. Protein A beads were crosslinked to the serum.
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Western blot - Anti-53BP1 antibody (ab172580)All lanes : Anti-53BP1 antibody (ab172580)
Lane 1 : U2OS (Control si)
Lane 2 : U2OS (53BP1 si)
Lane 3 : NIH 3T3 cell lysate at 40 µg
Lane 4 : PC12 cell lysate at 40 µg
Predicted band size: 214 kDa
Protocols
Datasheets and documents
References (14)
ab172580 has been referenced in 14 publications.
- Alonso-de Vega I et al. PHF2 regulates homology-directed DNA repair by controlling the resection of DNA double strand breaks. Nucleic Acids Res N/A:N/A (2020). WB ; Human . PubMed: 32232336
- Takasugi M et al. Naked mole-rat very-high-molecular-mass hyaluronan exhibits superior cytoprotective properties. Nat Commun 11:2376 (2020). ICC/IF ; Human . PubMed: 32398747
- Guo G et al. miR-7/SP1/TP53BP1 axis may play a pivotal role in NSCLC radiosensitivity. Oncol Rep 44:2678-2690 (2020). WB ; Human . PubMed: 33125142
- Tian X et al. SIRT6 Is Responsible for More Efficient DNA Double-Strand Break Repair in Long-Lived Species. Cell 177:622-638.e22 (2019). IF ; Mouse, Hamster, Gerbil, Other species, Porcupine . PubMed: 31002797
- Werbrouck C et al. TP53 pathway alterations drive radioresistance in Diffuse Intrinsic Pontine Gliomas (DIPG). Clin Cancer Res N/A:N/A (2019). ICC/IF ; Human . PubMed: 31481512
