Product nameAnti-53BP1 antibody
See all 53BP1 primary antibodies
DescriptionRabbit polyclonal to 53BP1
Tested applicationsSuitable for: WB, ICC/IF, IP, Flow Cyt, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human 53BP1 aa 1400-1700.
Storage instructionsShipped at 4°C. Store at -20°C.
Storage bufferConstituent: Water
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab172580 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 214 kDa.|
|IP||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionMay have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.
Involvement in diseaseNote=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein.
Sequence similaritiesContains 2 BRCT domains.
modificationsAsymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation.
Cellular localizationNucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks.
- Information by UniProt
- 53 BP1 antibody
- 53BP1 antibody
- FLJ41424 antibody
Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling 53BP1 with ab172580 at 1:1000. Cells were fixed with PFA and permeabilized with methanol for 2 minutes. Cells were incubated with the primary anitbody overnight at room temperature. Top - 53BP1, Middle - DAPI, Bottom - Merge.
Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling 53BP1 with ab172580 at 1:1000. Cells were fixed with PFA and permeabilized with methanol for 2 minutes. Cells were incubated with the primary anitbody overnight at room temperature.
Flow Cytometry analysis of HeLa cells labelling 53BP1 with ab172580 at 1:800. Cells were fixed with 1% PFA. Cells were incuabted with the primary antibody overnight at 4°C.
Immunohistochemistry (formalin/PFA-fixed paraffin-embedded) analysis of Human linfoid tissue labelling 53BP1 with ab172580 at 1:1000. Antigen retrieval was by microwave boiling for 7 minutes in 10mM TrisHCl, pH9.
Immunoprecipitation analysis of U2OS cells labelling 53BP1 with ab172580. Protein A beads were crosslinked to the serum.
All lanes : Anti-53BP1 antibody (ab172580)
Lane 1 : U2OS (Control si)
Lane 2 : U2OS (53BP1 si)
Lane 3 : NIH 3T3 cell lysate at 40 µg
Lane 4 : PC12 cell lysate at 40 µg
Predicted band size: 214 kDa
This product has been referenced in:
- Tian X et al. SIRT6 Is Responsible for More Efficient DNA Double-Strand Break Repair in Long-Lived Species. Cell 177:622-638.e22 (2019). IF ; Mouse, Hamster, Gerbil, Other species, Porcupine . Read more (PubMed: 31002797) »
- Werbrouck C et al. TP53 pathway alterations drive radioresistance in Diffuse Intrinsic Pontine Gliomas (DIPG). Clin Cancer Res N/A:N/A (2019). ICC/IF ; Human . Read more (PubMed: 31481512) »