Product nameAnti-53BP1 antibody
See all 53BP1 primary antibodies
DescriptionRabbit polyclonal to 53BP1
SpecificityThis antibody is specific for 53BP1.
Tested applicationsSuitable for: IP, Flow Cyt, WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide representing a portion of human 53BP1 encoded in part by exons 11 and 12 (LocusLink ID 7158).
- U205, HeLa, 293T, MO59K cell lysates (human) and MEF lysates (mouse).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab36823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|Flow Cyt||Use a concentration of 1.5 µg/ml.
1.5 µg/ml in a 150 mcl reaction.
|WB||1/10000 - 1/25000. Detects a band of approximately 220 kDa (predicted molecular weight: 214 kDa).|
|ICC/IF||1/100 - 1/500.|
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.
Involvement in diseaseNote=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein.
Sequence similaritiesContains 2 BRCT domains.
modificationsAsymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation.
Cellular localizationNucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks.
- Information by UniProt
- 53 BP1 antibody
- 53BP1 antibody
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal cell carcinoma tissue labelling 53BP1 with ab36823 at 1/1000 (1µg/ml). Detection: DAB.
All lanes : Anti-53BP1 antibody (ab36823) at 1/1000 dilution
Lane 1 : U2OS cell lysate
Lane 2 : 293T cell lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Predicted band size: 214 kDa
Observed band size: 220 kDa why is the actual band size different from the predicted?
Exposure time: 5 seconds
Ab36823, at a dilution of 1/100, staining 53BP1 foci in proliferating MEFs, both normal and exposed to 10 Gy of IR and double color immunofluorescence. Staining was for 2 hours.
Ab36823, at a dilution of 1/100, staining 53BP1 foci in proliferating MO59K cells, both normal and exposed to 10 Gy of IR and double color immunofluorescence. Staining was for 2 hours.
Flow Cytometry: 53BP1 Antibody [ab36823] - Flow cytometric detection of 53bp1. 1 million Jurkat cells were fixed, permeabilized, and stained with 1.5 mcg/ml anti-53bp1 ab36823 in a 150 mcl reaction. Isotype control (black), anti-53bp1 (red).
This product has been referenced in:
- Page BDG et al. Targeted NUDT5 inhibitors block hormone signaling in breast cancer cells. Nat Commun 9:250 (2018). Read more (PubMed: 29343827) »
- Yang Y et al. Nuclear GSK3ß induces DNA double-strand break repair by phosphorylating 53BP1 in glioblastoma. Int J Oncol 52:709-720 (2018). Read more (PubMed: 29328365) »