• Product name
  • Description
    Rabbit polyclonal to 53BP1
  • Host species
  • Specificity
    This antibody is specific for 53BP1 protein.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Cow, Dog, Chimpanzee, Baboon, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide representing a portion of human 53BP1 encoded exon 19 (LocusLink ID 7158).

  • Positive control
    • U205, 293T and MO59K cell lysates (human). MEF lysates (mouse).



Our Abpromise guarantee covers the use of ab36828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 220 kDa (predicted molecular weight: 214 kDa).
ICC/IF 1/200 - 1/1000.


  • Function
    May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.
  • Involvement in disease
    Note=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein.
  • Sequence similarities
    Contains 2 BRCT domains.
  • Post-translational
    Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
    Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation.
  • Cellular localization
    Nucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks.
  • Information by UniProt
  • Database links
  • Alternative names
    • 53 BP1 antibody
    • 53BP1 antibody
    • FLJ41424 antibody
    • MGC138366 antibody
    • p202 antibody
    • p53 binding protein 1 antibody
    • p53 BP1 antibody
    • p53-binding protein 1 antibody
    • p53BP1 antibody
    • TP53 BP1 antibody
    • TP53B_HUMAN antibody
    • Tp53bp1 antibody
    • TRP53 BP1 antibody
    • Tumor protein 53 binding protein 1 antibody
    • Tumor protein p53 binding protein 1 antibody
    • Tumor suppressor p53 binding protein 1 antibody
    • Tumor suppressor p53-binding protein 1 antibody
    see all


  • Anti-53BP1 antibody (ab36828) at 1/2000 dilution + Whole cell lysate from human U205 cells at 20 µg

    Developed using the ECL technique.

    Predicted band size: 214 kDa
    Observed band size: 220 kDa (why is the actual band size different from the predicted?)

    The arrow next to the image points to 53BP1 resolved on a 3 to 8% trisacetate gel.


This product has been referenced in:
  • Han W  et al. Nitric oxide mediated DNA double strand breaks induced in proliferating bystander cells after alpha-particle irradiation. Mutat Res 684:81-9 (2010). ICC/IF ; Chinese hamster . Read more (PubMed: 20026341) »

See 1 Publication for this product

Customer reviews and Q&As

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