Recombinant Anti-53BP1 antibody [BL-250-1H11] (ab243868)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BL-250-1H11] to 53BP1
- Suitable for: IP, WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-53BP1 antibody [BL-250-1H11]
See all 53BP1 primary antibodies -
Description
Rabbit monoclonal [BL-250-1H11] to 53BP1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human 53BP1 aa 350-400. The exact sequence is proprietary. NP_005648.1 and Gene ID 7158.
Database link: Q12888 -
Positive control
- WB: HeLa, HEK-293T, MCF7, HepG2, A549, SW620, SK-MEL-28, Jurkat whole cell lysates. IHC: mouse renal cell carcinoma, human colon carcinoma IP: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
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General notes
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA -
Concentration information loading...
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Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BL-250-1H11 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab243868 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
Use 20µl/mg lysate. |
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WB |
1/1000.
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IHC-P |
1/100 - 1/500.
Epitope retrieval with citrate buffer pH6.0 is recommended for Formalin-fixed, paraffin-embedded tissue sections |
Notes |
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IP
Use at an assay dependent concentration. Use 20µl/mg lysate. |
WB
1/1000. |
IHC-P
1/100 - 1/500. Epitope retrieval with citrate buffer pH6.0 is recommended for Formalin-fixed, paraffin-embedded tissue sections |
Target
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Function
May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage. -
Involvement in disease
Note=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein. -
Sequence similarities
Contains 2 BRCT domains. -
Post-translational
modificationsAsymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation. -
Cellular localization
Nucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks. - Information by UniProt
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Database links
- Entrez Gene: 7158 Human
- Entrez Gene: 27223 Mouse
- Omim: 605230 Human
- SwissProt: Q12888 Human
- SwissProt: P70399 Mouse
- Unigene: 440968 Human
- Unigene: 383499 Mouse
- Unigene: 481841 Mouse
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Alternative names
- 53 BP1 antibody
- 53BP1 antibody
- FLJ41424 antibody
see all
Images
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Western blot analysis using ab243868 at 1/1000 dilution.
Lane 1: HeLa whole cell lysate (15 µg).
Lane 2: HEK-293T whole cell lysate (15 µg).
Lane3: MCF7 whole cell lysate (15 µg).
Lane 4: HepG2 whole cell lysate (15 µg).
Lane 5: A549 whole cell lysate (15 µg).
Lane 6: SW620 whole cell lysate (15 µg).
Lane 7: SK whole cell lysate (15 µg).
Lane 8: Jurkat whole cell lysate (15 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 10 seconds
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53BP1 was immunoprecipitated from 1 mg HEK-293T whole cell lysate with ab243868 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243868 at 1/1000 dilution.
Lane 1: ab243868 IP in HEK-293T cell lysate.
Lane 2: Contol IgG in HEK-293T cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
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Formalin-fixed, paraffin-embedded mouse renal cell carcinoma stained using ab243868 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
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Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained using ab243868 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab243868 has not yet been referenced specifically in any publications.