Overview

  • Product name

    Anti-53BP1 antibody [BL-250-1H11]
    See all 53BP1 primary antibodies
  • Description

    Rabbit monoclonal [BL-250-1H11] to 53BP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human 53BP1 aa 350-400. The exact sequence is proprietary. NP_005648.1 and Gene ID 7158.
    Database link: Q12888

  • General notes

    This product is sold under License from Bethyl Laboratories, Inc.

Properties

Applications

Our Abpromise guarantee covers the use of ab243868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.

Use 20µl/mg lysate.

WB 1/1000.

Target

  • Function

    May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.
  • Involvement in disease

    Note=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein.
  • Sequence similarities

    Contains 2 BRCT domains.
  • Post-translational
    modifications

    Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
    Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation.
  • Cellular localization

    Nucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks.
  • Information by UniProt
  • Database links

  • Alternative names

    • 53 BP1 antibody
    • 53BP1 antibody
    • FLJ41424 antibody
    • MGC138366 antibody
    • p202 antibody
    • p53 binding protein 1 antibody
    • p53 BP1 antibody
    • p53-binding protein 1 antibody
    • p53BP1 antibody
    • TP53 BP1 antibody
    • TP53B_HUMAN antibody
    • Tp53bp1 antibody
    • TRP53 BP1 antibody
    • Tumor protein 53 binding protein 1 antibody
    • Tumor protein p53 binding protein 1 antibody
    • Tumor suppressor p53 binding protein 1 antibody
    • Tumor suppressor p53-binding protein 1 antibody
    see all

Images

  • 53BP1 was immunoprecipitated from 1 mg HEK-293T whole cell lysate with ab243868 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243868 at 1/1000 dilution.

    Lane 1: ab243868 IP in HEK-293T cell lysate.

    Lane 2: Contol IgG in HEK-293T cell lysate.

    Detection: Chemiluminescence with an exposure time of 10 seconds.

  • Western blot analysis using ab243868 at 1/1000 dilution.

    Lane 1: HeLa whole cell lysate (15 µg).

    Lane 2: HEK-293T whole cell lysate (15 µg).

    Lane3: MCF7 whole cell lysate (15 µg).

    Lane 4: HepG2 whole cell lysate (15 µg).

    Lane 5: A549 whole cell lysate (15 µg).

    Lane 6: SW620 whole cell lysate (15 µg).

    Lane 7: SK whole cell lysate (15 µg).

    Lane 8: Jurkat whole cell lysate (15 µg).

    A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 10 seconds

References

ab243868 has not yet been referenced specifically in any publications.

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